SN/T 4675.14-2016Determination of natamycin in wine for export--LC-MS/MS method (English PDF)

Foreword

SN/T 4675 "Methods for Quality and Safety of Exported Wines" is divided into 30 parts.

---SN/T 4675.1 Determination of glycerol in exported wines;

---SN/T 4675.2 Determination of 2,3-butanediol in exported wines by gas chromatography;

---SN/T 4675.3 Determination of the stable carbon isotope ratio of ethanol in exported wines;

---SN/T 4675.4 Determination of lactic acid in exported wines;

--- SN/T 4675.5 Determination of organic acids in exported wines by ion chromatography;

---SN/T 4675.6 Determination of glucose, fructose and sucrose in exported wines;

--- SN/T 4675.7 Determination of acetaldehyde in exported wines by gas chromatography-mass spectrometry;

--- SN/T 4675.8 Determination of 5-hydroxymethylfurfural in exported wines by liquid chromatography;

--- SN/T 4675.9 Determination of diethylene glycol in exported wines by gas chromatography-mass spectrometry;

---SN/T 4675.10 Determination of ochratoxin A in exported wines by liquid chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.11 Determination of seven anthocyanins in exported wines by ultra performance liquid chromatography;

---SN/T 4675.12 Determination of lysozyme in exported wines by liquid chromatography;

--- SN/T 4675.13 Determination of 2,4,6-trichloroanisole residues in exported wines by gas chromatography-mass spectrometry;

---SN/T 4675.14 Determination of natamycin in exported wines by liquid chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.15 Determination of salicylic acid, dehydroacetic acid and p-chlorobenzoic acid in exported wines by liquid chromatography;

---SN/T 4675.16 Determination of fumaric acid in exported wines by liquid chromatography-mass spectrometry/mass spectrometry;

--- SN/T 4675.17 Determination of butyl tin content in exported wines by gas chromatography - mass spectrometry/mass spectrometry;

--- SN/T 4675.18 Determination of residual dithiocarbamate in exported wines.

---SN/T 4675.19 Determination of sodium, magnesium, potassium, calcium, chromium, manganese, iron, copper, zinc, arsenic, selenium, silver, cadmium and lead in exported wines;

---SN/T 4675.20 Determination of rare earth elements in exported wines by inductively coupled plasma mass spectrometry;

--- SN/T 4675.21 Determination of soluble inorganic salts in exported wines by ion chromatography;

---SN/T 4675.22 Determination of total sulfur dioxide in exported wines;

---SN/T 4675.23 Determination of ammonia nitrogen in exported wines and grape juices - Continuous flow analyzer method;

---SN/T 4675.24 Determination of the export wine Fulin-Shoka index spectrophotometer method;

--- SN/T 4675.25 Determination of the color of exported wine CIE1976 (L * a * b *) color space method;

---SN/T 4675.26 Determination of turbidity of exported wines by scattered light method;

---SN/T 4675.27 Determination of alkaline ash in exported wines;

---SN/T 4675.28 Counting bacteria, mold and yeast for exporting wine;

---SN/T 4675.29 Real-time fluorescent PCR method for wine yeast test in exported wine;

---SN/T 4675.30 In the wine, the Bayesian yeast test real-time fluorescent PCR method.

This part is the 14th part of SN/T 4675.

This part is drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This part of SN/T 4675 specifies a method for the determination of natamycin in exported wine by liquid chromatography-tandem mass spectrometry.

This section applies to the determination of natamycin in wine.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.

Pieces. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

3 Method summary

The sample was diluted, centrifuged at high speed, and determined by liquid chromatography-mass spectrometry/mass spectrometry and quantified by external standard method.

4 Reagents and materials

Unless otherwise stated, the reagents used are of excellent grade and the water is the first grade water specified in GB/T 6682.

4.1 Acetonitrile. chromatographically pure.

4.2 Formic acid.

4.3 5% acetonitrile-0.1% formic acid solution. Accurately measure 50mL acetonitrile, 1mL formic acid, dilute to 1L with water, mix and use.

4.4 0.1% formic acid-water solution. Accurately measure 1 mL of formic acid, dilute to 1 L with water, mix well and set aside.

4.5 Natamycin standard (CAS number 7681-93-8, molecular weight. 665.73). purity >= 89.9%.

4.6 Natamycin standard stock solution. accurately weigh the appropriate amount of natamycin standard, and use methanol to prepare a standard of 1.0mg/mL.

Stock solution.

4.7 Natamycin standard intermediate solution. Accurately absorb the appropriate amount of natamycin standard stock solution (4.6) with 5% acetonitrile-0.1% formic acid solution (4.3) A standard intermediate solution having a concentration of 10 µg/mL was prepared.

4.8 Natamycin standard working solution. Accurately absorb the appropriate amount of natamycin standard intermediate solution (4.7), using 5% acetonitrile-0.1% formic acid solution (4.3) The natamycin standard intermediate solution was diluted stepwise to prepare a standard working solution of 0.2 µg/L to 100 µg/L.

5 Instruments and equipment

5.1 High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometer. Triple Quadrupole Mass Spectrometry with Electrospray Ion Source (ESI).

5.2 Analytical balance. Sensing amount 0.01mg.

5.3 Vortex mixer.

5.4 Centrifuge. equipped with 2mL angle rotor, the speed is not less than 15000r/min.

5.5 stoppered centrifuge tube. 50mL, 2mL.