SN/T 3872-2014Detection of four pathogens in food for export. MALDI-TOF-MS method (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine.

1 Scope

This standard specifies the MALDI-TOF-MS detection of four pathogenic bacteria in food.

This standard applies to food Salmonella, Listeria monocytogenes bacteria, Vibrio parahaemolyticus, Vibrio cholerae four kinds of pathogens Rapid detection of other pathogens can refer to use.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

National Food Safety Standard GB 4789.4 Microbiological Examination of Salmonella food GB 4789.7 national food safety standards of food microbiology testing Vibrio parahaemolyticus test National Food Safety Standard GB 4789.30 Microbiological examination of food monocytogenes Listeria test Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety SN 0170 export food Salmonella (bacteria including Arizona) test method SN/T 0173 Importers and Vibrio parahaemolyticus in food testing methods SN/T 0184.1 import and export food monocytogenes Listeria detection methods SN/T 1022 Vibrio cholerae in food import and export inspection methods NMKLNo.156 food in the detection and enumeration of Vibrio

3 Abbreviations

The following abbreviations apply to this document.

4 Method summary

MALDI-TOF-MS is applied to the rapid detection of bacterial whole cell technology, based primarily on features present in the bacterial surface proteins Molecular weight information, the completion of the identification and classification of bacteria. Dot matrix solution will be identified colonies sample with a suitable amount of added sample plate, the solvent evaporated After the formation of hair sample matrix co-crystallized by laser radiation as an energy source co-crystals, matrix molecules absorb energy and sample desorption