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SN/T 3678-2013Detection and identification of Tilletia puccinelliae Carris, Castl. and G.Huang. (English PDF)

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Issued by

General Administration of Customs

Level / Type

National · Recommended

Issue date

August 30, 2013

Implementation date

March 1, 2014

Scope

SN/T 3678-2013 (Detection and identification of Tilletia puccinelliae Carris, Castl. and G.Huang.) is available as an English-translated PDF.

SN/T 3678-2013 - This standard specifies the Puccinillia Tilletia fungus (TilletiapuccinelliaeCarris, Castl. State determination, germination experiment steps and methods.

This standard applies to quarantine and identification Puccinillia seeds Puccinillia Tilletia bacteria.

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Document preview — SN/T 3678-2013

National Standard of the People's Republic of China

Issued by: General Administration of Customs

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Puccinillia Tilletia fungus basic information
  • 4 Principle of the method
  • 5 Equipment and reagents
  • 5.1 Equipment
  • 5.2 Reagents and culture medium

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.

1 Scope

This standard specifies the Puccinillia Tilletia fungus (TilletiapuccinelliaeCarris, Castl.

State determination, germination experiment steps and methods.

This standard applies to quarantine and identification Puccinillia seeds Puccinillia Tilletia bacteria.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 18085-2000 phytosanitary wheat dwarf bunt quarantine and identification of bacteria

3 Puccinillia Tilletia fungus basic information

Scientific name. TilletiapuccinelliaeCarris, Castl.

Chinese name. Puccinillia Tilletia fungus Taxonomic position. Puccinillia fishy Ustilago Fungi (Fungi), Basidiomycota (Basidiomycota), Ustilago Gang (Ustilaginomyce-tes), outside Basidiomycetes subclass (Exobasidiomycetidae), fishy Ustilago mesh (Tiletiales), fishy ustilaginaceae (Tiletiaceae), Tilletia Genus (Tilletia).

Host. Puccinillia Puccinelliadistans (L.) Parl.

Distribution. United States

4 Principle of the method

Germs and bacteria teliospore gall morphology, physiological characteristics of winter spore germination (see Appendix A) is an alkali Mao fungus Tilletia quarantine identification basis.

5 Equipment and reagents

5.1 Equipment

Magnifier, sterile petri dish (diameter 9cm), flasks, centrifuge tubes, centrifuge tubes (1.5 mL of), tweezers, alcohol lamp, slides, cover glass Tablets.

Low-speed centrifuge, reciprocating oscillator, a vortex, stereo microscope, biological microscope, clean benches, biological incubator, electronic the balance (inductance 0.01g), Autoclave like.

5.2 Reagents and culture medium

Tween -20 (Tween-20), Schiel's floating carrier (see GB/T 18085-2000 in A Schiel's floating carrier preparation method appendix), Joan Fat, 0.25% sodium hypochlorite (NaClO4), absolute ethanol.

3% water agar.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 7 pages — is available in the English PDF.

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