SN/T 3639-2013Determination of Campylobacter jejuni in food for export. Real-time PCR method (English PDF)
出口食品中空肠弯曲菌的检测方法 实时荧光PCR法
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Issued by
General Administration of Customs
Level / Type
National · Recommended
Issue date
August 30, 2013
Implementation date
March 1, 2014
Scope
SN/T 3639-2013 is the English-translated version of 出口食品中空肠弯曲菌的检测方法 实时荧光PCR法.
SN/T 3639-2013 - This standard specifies the foods Campylobacter jejuni (Campylobacterjejuni) of real-time PCR method. This standard applies to foods genetic testing of C. jejuni.
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Document preview — SN/T 3639-2013
National Standard of the People's Republic of China
Issued by: General Administration of Customs
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Anti-pollution measures
- 4 Experimental Method
- 4.1 Equipment and Materials
- 4.2 Reagents
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, Chinese Center for Disease Control and Prevention, the People's Republic of China State Tianjin Entry-Exit Inspection and Quarantine Beijing People's Republic of China, People's Republic of China Fujian Entry-Exit Inspection and Quarantine Bureau, Guangxi People's Republic of China Entry-Exit Inspection and Quarantine of Jilin People's Republic of China, People's Republic of China State Hunan Entry-Exit Inspection and Quarantine People's Republic of China, Shandong, Shanghai Hui Rui Biotechnology Limited.
1 Scope
This standard specifies the foods Campylobacter jejuni (Campylobacterjejuni) of real-time PCR method.
This standard applies to foods genetic testing of C. jejuni.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 4789.9 Microbiological examination of food hygiene inspection Campylobacter jejuni Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety Campylobacter detection method SN/T 0175 imported and exported food SN/T 1193 genetic testing laboratory technical requirements
3 Anti-pollution measures
Pollution prevention measures inspection process should be consistent with SN/T 1193.
4 Experimental Method
4.1 Equipment and Materials
4.1.1 real-time PCR instrument.
4.1.2 clean bench.
4.1.3 Sterilization pot.
4.1.4 ice machine.
4.1.5 nucleic acid protein analyzer.
4.1.6 High-speed refrigerated centrifuge. speed >=8000r/min; small desktop centrifuge. speed >=13000r/min.
4.1.7 low temperature freezer, refrigeration refrigerator.
4.1.8 Water, double distilled water heaters.
4.1.9 Vortex.
4.1.10 micropipette.
4.1.11 No Dnase/Rnase optical PCR reaction tubes or plates.
4.2 Reagents
Unless otherwise specified, all reagents were of analytical grade or biochemical reagents.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 4 pages — is available in the English PDF.
Referenced standards
Normative references
- GB/T 4789.9Microbiological examination of food hygiene - Examination of campylobacter jejuni
- GB/T 6682Water for analytical laboratory use - Specification and test methods
- GB 19489Laboratories - General requirements for biosafety
- SN/T 0175Detection of Campylobacter spp.in food for import and export
SN/T 1193
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Related Standards
GB 19489-2004 — Laboratories - General requirements for biosafety
GB 19489-2008 — Laboratories - General requirements for biosafety
GB/T 4789.9-2003 — Microbiological examination of food hygiene - Examination of Campylobacter jejuni
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SN/T 3639-2013
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