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SN/T 3639-2013Determination of Campylobacter jejuni in food for export. Real-time PCR method (English PDF)

出口食品中空肠弯曲菌的检测方法 实时荧光PCR法

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Issued by

General Administration of Customs

Level / Type

National · Recommended

Issue date

August 30, 2013

Implementation date

March 1, 2014

Scope

SN/T 3639-2013 is the English-translated version of 出口食品中空肠弯曲菌的检测方法 实时荧光PCR法.

SN/T 3639-2013 - This standard specifies the foods Campylobacter jejuni (Campylobacterjejuni) of real-time PCR method. This standard applies to foods genetic testing of C. jejuni.

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Document preview — SN/T 3639-2013

National Standard of the People's Republic of China

Issued by: General Administration of Customs

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Anti-pollution measures
  • 4 Experimental Method
  • 4.1 Equipment and Materials
  • 4.2 Reagents

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, Chinese Center for Disease Control and Prevention, the People's Republic of China State Tianjin Entry-Exit Inspection and Quarantine Beijing People's Republic of China, People's Republic of China Fujian Entry-Exit Inspection and Quarantine Bureau, Guangxi People's Republic of China Entry-Exit Inspection and Quarantine of Jilin People's Republic of China, People's Republic of China State Hunan Entry-Exit Inspection and Quarantine People's Republic of China, Shandong, Shanghai Hui Rui Biotechnology Limited.

1 Scope

This standard specifies the foods Campylobacter jejuni (Campylobacterjejuni) of real-time PCR method.

This standard applies to foods genetic testing of C. jejuni.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 4789.9 Microbiological examination of food hygiene inspection Campylobacter jejuni Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety Campylobacter detection method SN/T 0175 imported and exported food SN/T 1193 genetic testing laboratory technical requirements

3 Anti-pollution measures

Pollution prevention measures inspection process should be consistent with SN/T 1193.

4 Experimental Method

4.1 Equipment and Materials

4.1.1 real-time PCR instrument.

4.1.2 clean bench.

4.1.3 Sterilization pot.

4.1.4 ice machine.

4.1.5 nucleic acid protein analyzer.

4.1.6 High-speed refrigerated centrifuge. speed >=8000r/min; small desktop centrifuge. speed >=13000r/min.

4.1.7 low temperature freezer, refrigeration refrigerator.

4.1.8 Water, double distilled water heaters.

4.1.9 Vortex.

4.1.10 micropipette.

4.1.11 No Dnase/Rnase optical PCR reaction tubes or plates.

4.2 Reagents

Unless otherwise specified, all reagents were of analytical grade or biochemical reagents.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 4 pages — is available in the English PDF.

Referenced standards

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