SN/T 3577-2013Detection of genetically modified components. Method for cotton using PCR-DHPLC (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, China Inspection Quarantine Science Research Institute, Shenzhen Bo Rui Xiang Hui Biotechnology Co., Ltd., National Center for Nanoscience.

1 Scope

This standard specifies the entry and exit of genetically modified cotton and PCR-DHPLC detection lines.

The standard method is suitable for the detection of genetically modified cotton line MON531, MON1445, MON1698, MON15985 in transgenic Due to component and line detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.7 GMO detection methods of sampling and sample preparation SN/T 1193 genetic testing laboratory technical requirements Principle 3 Denaturing high-performance liquid chromatography (denaturinghigh-performanceliquidchromatography, DHPLC) is a simple Single, rapid, nucleic acid analysis method of non-gel at 50 °C condition of sample analysis, the number of base pairs in the sample determines the order of elution peak, when over Column acetonitrile concentration increases, the nucleic acid fragment will be from small to large order was eluted according to the relative molecular mass. This standard uses the multiplex PCR Methods for transgenic cotton containing genetically modified to amplify the same time, the amplified products were analyzed by DHPLC. By DHPLC the elution peak was determined by comparison with molecular weight marker size, it determines whether or not with a foreign gene and determine the composition of genetically modified cotton strain.

4 Equipment, utensils and reagents