SN/T 3503-2013Rapid screen of ractopamine residues with nano magnetic particles. Immunechromatographic assay (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

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This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, Tsinghua University Shenzhen Graduate School, Shenzhen Key Laboratory of harmful alien biomass detection technology development.

1 Scope

This standard specifies the use of nano-magnetic immunity chromatography, foods of animal origin, feed and urine ractopamine residues fast Screening assays.

This standard applies to foods of animal origin, feed and urine samples ractopamine residues rapid screening qualitative detection.

The standard detection limit of 0.1µg/kg.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 14699.1 feed samples GB/T 18088 Entry and Exit Animal Quarantine sampling SN/T 2123 Entry and Exit Animal Quarantine experimental sample collection, transport and storage specifications Principle 3 in this method, immunochromatography the competitive inhibition method, its main principle is. by bovine serum albumin conjugate ractopamine antigen, Anti-mouse IgG antibody, nano superparamagnetic-labeled anti-ractopamine antibodies and other reagents immobilized in a nitrocellulose membrane, a cellulose membrane glass and other membranes made of paper test strip, the application of the principles of competition law formula chromatography test sample ractopamine residues. Samples Laikeduoba Amine in the process of flowing nano-cellulose membrane with glass beads labeled specific anti-ractopamine antibody binding, and antibody inhibited nitrate Acid cellulose membrane test line BSA - binding antigen conjugate ractopamine, the detection line bound nanometer beads minus Less, is inversely proportional to the intensity of the magnetic resonance signals ractopamine content sample using super paramagnetic resonance detector measurement is in.

4 Reagents and Instruments