SN/T 3484-2013Quarantine protocol for ovine pulmonary adenocarcinoma (English PDF)
绵羊肺腺瘤病检疫技术规范
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Issued by
General Administration of Customs
Level / Type
National · Recommended
Issue date
March 1, 2013
Implementation date
September 16, 2013
Scope
SN/T 3484-2013 is the English-translated version of 绵羊肺腺瘤病检疫技术规范.
SN/T 3484-2013 - This standard specifies the clinical diagnosis and PCR assay method sheep pulmonary neoplasia. This standard applies to sheep pulmonary adenomatosis quarantine.
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Document preview — SN/T 3484-2013
National Standard of the People's Republic of China
- ICS
- 11.220
- Classification
- B41
Issued by: General Administration of Customs
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Abbreviations
- 4 Clinical diagnosis
- 4.1 Clinical symptoms
- 4.2 Pathological changes
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Heilongjiang People's Republic of China Exit Inspection and Quarantine.
1 Scope
This standard specifies the clinical diagnosis and PCR assay method sheep pulmonary neoplasia.
This standard applies to sheep pulmonary adenomatosis quarantine.
2 Normative references
The following documents for the application of this document is essential, dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1193 genetic testing laboratory technical requirements SN/T 2123 Entry and Exit Animal Quarantine experimental sample collection, transport and storage specifications
3 Abbreviations
The following abbreviations apply to this document.
Fluorescent signal cycle number reaches a set threshold when experienced. Ct values EDTA. sodium edetate (ehylenediamineteraaceticacid) JSRV. sheep pulmonary adenoma virus OPA. sheep pulmonary adenomatosis PCR. polymerase chain reaction Real-TimePCR. real-time PCR Taq. TaqDNA polymerase (TaqDNApolymerase)
4 Clinical diagnosis
4.1 Clinical symptoms
The main symptom is a progressive respiratory disorders, especially after exercise, breathing difficulty is obvious. Reaction severity of symptoms of lung tumor development degree. OPA respiratory effusion is the most typical symptoms, it is easy to check by auscultation to crackles. Bing Yang lift the hind legs, looking down, nose Hole out of a lot of foamy mucus, this is a typical "small carts test (wheel-barrowtest)", the characteristics of a typical diagnostic significance Righteousness. The early course of the disease, cough and anorexia are not common, but once obvious clinical symptoms, body weight showed a progressive decline. Course of several weeks or months After, often due to bacterial pneumonia associated with death, especially hemolytic mansoni bacilli (formerly known as Pasteurella). The outer part of the clinical sick animals Zhou lymphopenia (mainly CD4 and T lymphocytes and reduce neutrophils) phenomenon, which may help Pro Clinical diagnosis.
4.2 Pathological changes
4.2.1 Necropsy
Most cases of OPA lesions confined to the lungs, but can also occur in the chest, outside, transfer to lymph nodes and other tissues. Typical disease
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 8 pages — is available in the English PDF.
Referenced standards
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Related Standards
SN/T 1193-2003 — General requirements for the laboratories for gene detection and identification
SN/T 2123-2008 — Protocol of collection, transportation and storage of samples for entry and animal quarantine
SN/T 0330-2025 — General rules for microbiological examinations of export food
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