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SN/T 3304-2012Quarantine protocol for Babesiosis equine (English PDF)

马巴贝斯虫病检疫技术规范

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Issued by

General Administration of Customs

Level / Type

National · Recommended

Issue date

December 12, 2012

Implementation date

July 1, 2013

Scope

SN/T 3304-2012 is the English-translated version of 马巴贝斯虫病检疫技术规范.

SN/T 3304-2012 - This standard specifies Babesiosis equi pathogenic examination, indirect immunofluorescence assay, enzyme-linked immunosorbent assay, trace complement fixation Tests and technical requirements for the polymerase chain reaction method.

This standard applies to Babesiosis equi serological investigation, diagnosis and monitoring.

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Document preview — SN/T 3304-2012

National Standard of the People's Republic of China

Issued by: General Administration of Customs

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Abbreviations
  • 4 Clinical diagnosis
  • 5 Laboratory diagnosis
  • 5.1 Blood smear

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

This standard and the International Organization for Animal Health (OIE) "diagnostic tests and vaccines Standards Manual" (2010) in Chapter 2.5.8 the degree of consistency Non-equivalent.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Tianjin People's Republic of China Bureau, People's Republic of China Shandong CIQ.

1 Scope

This standard specifies Babesiosis equi pathogenic examination, indirect immunofluorescence assay, enzyme-linked immunosorbent assay, trace complement fixation Tests and technical requirements for the polymerase chain reaction method.

This standard applies to Babesiosis equi serological investigation, diagnosis and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 18088 Entry and Exit Animal Quarantine sampling SN/T 1526 Babesiosis equi trace detection methods complement fixation test methods

3 Abbreviations

The following abbreviations apply to this document.

B.equi.. Babesia equi EDTA. ethylenediaminetetraacetic acid FITC. fluorescein isothiocyanate

4 Clinical diagnosis

Refer to Appendix A, A.1 make a preliminary diagnosis.

5 Laboratory diagnosis

5.1 Blood smear

5.1.1 Reagents

5.1.1.1 EDTA solution (1mg/mL) (see Appendix A).

5.1.1.2 10% of Giemsa (see Appendix A).

5.1.1.3 methanol (AR).

5.1.2 Devices

5.1.2.1 optical microscope.

5.1.2.2 micrometer.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 10 pages — is available in the English PDF.

Referenced standards

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