SN/T 3170-2012Detection and identification of Xylella fastidiosa (English PDF)

3.1 Grape Pierce bacteria taxonomic status

Grape Pierce germs scientific name Xylellafastidiosa Welsetal, 1987, the genus Prokaryotae (Procaryotae), thin-walled Bacteria door (PhylumGracilicutes), xylem small genus (Xylella).

3.2 Based quarantine and identification

In bacteria characteristic symptoms on the host, pathogen morphological characteristics and cultural characteristics, as well as the characteristics of the bacteria species genome sequence as Portugal Pierce grapes quarantine pathogen identification based, if necessary, assisted Pathogenicity Test (see Appendix A).

4.1 Apparatus, equipment, utensils

Clean benches, biological incubator, electronic scales, microplate reader, PCR instrument, gel imager, water bath, high-speed refrigerated centrifuge, high pressure Autoclave, transmission electron microscopy, refrigerators, liquid nitrogen tanks, scalpel blades, white disk humidors, mortar, micropipette (adjustable range.

2.5µL ~ 1000µL), tip (10µL, 100µL, 1000µL), centrifuge tubes (1.5mL), PCR tubes, ELISA plate.

4.2 Reagents and culture medium

Test required medicines were of analytical grade.

Test reagent used in succinic acid - citrate - phosphate buffer, and its method of preparation see Appendix B.

PD2 used in the test medium, CS20 medium, PW medium, SPW medium, its method of preparation, see Appendix B.

5.1 Pairs antibody sandwich ELISA (DAS-ELISA) detection

The use of double-antibody sandwich enzyme-linked immunosorbent assay samples were screened (see Appendix C), if the test result is positive, the need for pathogens