SN/T 2910.3-2012Identification method of irradiated food for export. Part 3: GC/MS (English PDF)

2.1 Method summary

Using ethyl acetate as solvent extraction 2-dodecyl cyclobutanone, frozen fat by accelerated solvent extraction method, the SPE over a silica gel column Purification eluent was concentrated and dissolved constant volume, with the GC/MS instrument measured internal standard.

2.2 Reagents and materials

All reagents unless otherwise specified, were of analytical grade, water re-distilled water.

2.2.1 ethyl acetate.

2.2.2 ether.

2.2.3 n-hexane.

2.2.4 acetonitrile.

2.2.5 diatomaceous earth.

2.2.6 Silicone LC-Si SPE column. 1g, 6mL or equivalent person.

2.2.7 2-cyclohexyl-cyclohexanone standards (2-cyclohexylcyclohexanone, C12H20O, CASN o.90-42-6). purity >=98%.

2.2.8 2-dodecyl cyclobutanone standard (2-dodecylcyclobutanone, C16H30O, CASN o.35493-46-0). purity >=98%.

2.2.9 eluent. diethyl ether/n-hexane solution (298, volume ratio).

2.2.10 internal standard stock solution. weigh the amount of 2-cyclohexyl-cyclohexanone standards, formulated with n-hexane at a concentration of 1mg/mL standard Stock solution, set -18 °C refrigerator.

2.2.11 2-dodecyl cyclobutanone standard stock solution. Weigh the appropriate amount of 2-dodecyl-butanone standards, formulated in a concentration of n-hexane Standard stock solution 1mg/mL, and set -18 °C refrigerator.

2.2.12 internal standard working solution. Apply the internal standard stock solution was diluted with n-hexane was 0.5µg/mL of 2-cyclohexyl-cyclohexanone working fluid, Set -18 °C refrigerator.

2.2.13 2-dodecyl cyclobutanone standard working solution. take the appropriate amount of 2-alkyl cyclobutanone stock solution was diluted with n-hexane was 1.0µg/mL work for liquid, set -18 °C refrigerator.