SN/T 2215-2008Determination of phenothiazines residues in animal original food for for import and export. Enzyme-linked immunosorbent assay (English PDF)

Foreword

Appendix A of this standard is an informative annex.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.

1 Scope

This standard specifies the method of enzyme-linked immunosorbent assay phenothiazines antibiotic residues in animal products.

This standard applies to meat, offal, fish and milk chlorpromazine, acepromazine, propionyl oxaprozin, and total residual trifluoroacetic oxaprozin Determination.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Preparation and preservation of samples 3

4 Method summary

The standard samples were extracted with acetonitrile and basic residues chlorpromazine, acepromazine, propionyl oxaprozin,, and trifluoroacetic oxaprozin. Microplate package There chlorpromazine antibody was added chlorpromazine standard or sample extract and the enzyme was after chlorpromazine or free extract residues and enzyme-labeled Chlorpromazine and antibody binding competitive. Chlorpromazine and remove unbound enzyme-labeled purified by washing, followed by addition of chromogenic substrate, using a microplate reader Absorbance was measured from the absorbance worth of content in the sample residue.

5 Reagents and materials

Unless otherwise indicated, all reagents were of analytical grade water as a water GB/T 6682 regulations.

5.1 phenothiazines detection kit (see Appendix A).

5.2 acetonitrile.

5.3 40% sodium hydroxide solution. Accurately weigh 40.0g of sodium hydroxide dissolved in water and dilute to 100mL.

5.4 Standard. chlorpromazine, purity >=98%; acepromazine, purity >=98%; propionyl oxaprozin, purity >=98%; oxaprozin, purity >=98%; three Fluorine oxaprozin, purity >=98%.