SN/T 2021-2007Protocol of quarantine for bovine anaplasmosis (English PDF)

Foreword

This standard is a reference to the International Organization for Animal Health (OIE) "ManualofDiagnostic, TestsandVaccinesforTerrestrial Animals "(2004 edition) CHAPTER2.3.7BOVINEANAPLASMOSIS, at home and abroad a large number of references and standards Bovine anaplasmosis research diagnostic method of drafting units prepared.

The Standard Appendix B, Appendix C, Appendix D is a normative appendix, Appendix A is an information appendix.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Central China Agricultural University.

1 Scope

This standard specifies the bovine anaplasmosis quarantine technical specifications, including smear microscopy, polymerase chain reaction, enzyme-linked immunosorbent assay detection Measuring method.

This standard applies to bovine anaplasmosis quarantine.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 18651 bovine anaplasmosis rapid agglutination detection method SN/T 1679 animal anaplasmosis micro complement fixation test procedures

3 Overview

Bovine anaplasmosis (Bovineanaplasmosis) by Anaplasma parasites in cattle red blood cells, causing fever, anemia, jaundice and progressive Weight loss, or even a disease death. The distribution of the disease, pathogen classification, the media, etc. See Appendix A.

Diagnosis of bovine anaplasmosis include clinical symptoms and pathological changes, pathogen identification and serum antibodies. Serological detection methods the complement fixation test (Complementfixationtest, CFT) and card agglutination test (Cardagglutinationtest, CAT) is the most commonly used method. CFT used for many years, the rules see SN/T 1679, but recent data confirm the diagnosis of persistent infection in CFT when the cow, the sensitivity is only 20%, most of the carriers of the pathogen can not be detected. Therefore, CFT confirmatory test for individual animals is not reliable of. The advantages of CAT is sensitive, rapid, both in the laboratory can also be carried out in the field, the test results can be reached in a few minutes. False negative rate it is very low, but non-specific reaction is a problem. It can be used as screening methods, operating procedures, see GB/T 18651. Competitive ELISA method in the detection sensitivity Infected animals, which greatly increase the sensitivity. Indirect ELISA, dot ELISA and fluorescent antibody test can also be used to detect antibodies specific, but Since an indirect fluorescent antibody test to detect the operation of their day limited number of samples, and there are non-specific fluorescence reaction, generally wish to vote Other serological methods.

4 Diagnosis