SN/T 1913-2007Identification of Coffee Berry Borer [Hypothenemus hampei (Ferrari)] (English PDF)

2.1 Taxonomic status

2.2 Transmission

Mainly through the coffee fruit (beans), seeds and packaging materials over long distances, spread mainly by close female adults in the process of looking for nesting sites with airflow fly spread, experimental record female can fly the farthest 345m.

2.3 Identification based

Morphological characteristics of the coffee fruit beetles and distribution, biological characteristics and route of transmission is based on the development of methods for identification of quarantine.

3.1 Instrument

Stereo microscope, magnifying glass, insects dissecting needle, scalpel, tweezers, micrometer, finger-shaped tube, three sets of slides, cover slips, grinding jar, White porcelain plate, label, rearing boxes, incubators and the like.

3.2 Reagents

Ethanol - Glycerin preservation solution (75% ethanol. glycerol = 1 ~ 100.0.5).

4.1 If sectional test

According to the moth-eaten food fruit habit to check whether the fruit wormholes, attention focused on whether the wormhole near the top of the fruit. The coffee fruit with a scalpel Taken to observe whether the tunnel borer brown, carefully check inside the larvae, pupae or adults.

4.2 Feeding Test

You will be found to have larvae or pupae bore fruit into a petri dish placed in boxes or rearing breeding incubator at a temperature of Concept 25 °C ~ 30 °C conditions Police, within 25d ~ 36d adult emergence can.

4.3 Adults, larvae and pupae taxidermy

Adult specimens taken needle inserted dry and save, and pay attention to anti-insect; larvae and pupae before soaking into the water to boil 1min ~ 2min straight worms Hard, with ethanol - glycerol solution in preservation.

4.4 Microscopy

The larva, pupa or adult specimens placed stereoscopic dissecting microscope identification.