SN/T 1743-2006Determination of allure red AC, carmosine, brillint blue FCF, sunset yellow FCF in food. High performance liquid chromatogarphic method (English PDF)

3.1 Method summary

Synthetic colorants in candy and beverages are extracted by polyamide adsorption, filtered, and then subjected to reversed-phase high performance liquid chromatography.

From the determination, the external standard method is quantified.

3.2 Reagents and materials

Unless otherwise stated, the reagents used were of analytical grade and the water was secondary deionized water.

3.2.1 Polyamide powder..200 mesh.

3.2.2 Ethanol-Ammonia solution. Take 1 mL of ammonia water and add 70% of 100 mL of ethanol.

3.2.3 Methanol-formic acid solution. Mix 60 mL of methanol and 40 mL of formic acid.

3.2.4 20% citric acid solution..200 g of citric acid was dissolved in 1 L of water.

3.2.5 Sulfuric acid. Mix 10mL of sulfuric acid and 100mL of water.

3.2.6 10% sodium tungstate aqueous solution. 10 g of sodium tungstate was dissolved in 100 mL of water.

3.2.7 Acetic acid. chromatographically pure.

3.2.8 Methanol. Chromatographically pure.

3.2.9 Ammonium acetate.

3.2.10 Sunset yellow, tempting red, bright blue, acid red standard. purity greater than 98%.

3.2.11 Standard solution. accurately weigh the appropriate amount of colorant standard, and prepare it into a standard stock solution of 1.0 mg/mL with water.

It should be diluted with water and formulated into a standard working solution of appropriate concentration.

3.3 Instruments and equipment

3.3.1 Liquid chromatograph with UV detector.

3.3.2 Vortex mixer.

3.3.3 Centrifuge. Maximum speed 5000r/min.

3.4 Analysis steps

3.4.1 Extraction, purification

3.4.1.1 Samples such as juice and soda. Heat 50mL sample to remove carbon dioxide, then dilute with water to make a volume of 0.45µm filter.