Valid

SN/T 1204-2003Protocol of the real-time PCR for detecting genetically modified plants and their derived products (English PDF)

Preview PDF

Also coversSNT1204-2003

This is a limited preview

Buy now to download the full PDF (8 pages)

Need quotation for your management?

Get a formal quotation for SN/T 1204-2003 in 30 minutes.

Issued by

General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China

Level / Type

National · Recommended

Issue date

July 17, 2008

Implementation date

February 1, 2009

Scope

SN/T 1204-2003 (Protocol of the real-time PCR for detecting genetically modified plants and their derived products) is available as an English-translated PDF.

SN/T 1204-2003 is the Chinese standard "Protocol of the real-time PCR for detecting genetically modified plants and their derived products". Its scope clause reads: This Standard specifies the liquid-chromatography determination for the methylisothiazolinone (MIT) and chloromethylisothiazolinone (CIT) in cosmetics.

This Standard is applicable to the determination of the methylisothiazolinone and chloromethylisothiazolinone in skin-cleaning cosmetics and skin-care cosmetics.

Its clauses include principle; reagents and materials; results calculation. It was issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China on 2008-07-17 and took effect on 2009-02-01.

Found what you need?

Document preview — SN/T 1204-2003

National Standard of the People's Republic of China

Issued by: General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China

Foreword...3
1 Scope ..4
2 Principle...4
3 Reagents and materials...4
4 Instruments...5
5 Determination procedure...5
6 Results calculation ..6
7 Low limit of the determination ..7
8 Recovery rate and precision...7
Appendix A (Informative) Liquid chromatogram of standard substance ..8

1 Scope

This Standard specifies the liquid-chromatography determination for the methylisothiazolinone (MIT) and chloromethylisothiazolinone (CIT) in cosmetics. This

Standard is applicable to the determination of the methylisothiazolinone and chloromethylisothiazolinone in skin-cleaning cosmetics and skin-care cosmetics.

2 Principle

Use ultrasound to extract the methylisothiazolinone and chloromethylisothiazolinone in cosmetics with methanol; Perform centrifugal filtration; adopt the high-performance liquid chromatography to conduct separation and determination. Use the retention time for qualitativeness; use the external standard method for quantification.

3 Reagents and materials

Unless otherwise stated, the reagents used shall be the analytical reagent; the water shall be the secondary deionized water.

3.1 Methanol. chromatographically pure reagent.

3.2 Methylisothiazolinone (2-methyl-4-isothiazolone-3-ketone). the purity is >= 98%.

3.3 Chloromethylisothiazolinone (5-chlorine-2-methyl-4-isothiazolone-3-ketone). the purity

is >= 98%.

3.4 Standard-stock mixed solution of methylisothiazolinone and

chloromethylisothiazolinone. accurately weigh (3.2) 0.025g of methylisothiazolinone and (3.3) 0.1g of chloromethylisothiazolinone respectively. The accuracy is 0.0001 g. Place them in the 50 mL beaker; add appropriate amount of methanol to dissolve it. Transfer quantitative solution in to the 100mL volumetric flask; dilute it with methanol to the scale;

mix it uniformly. In this solution, the concentration of methylisothiazolinone is 250mg/L; the concentration of chloromethylisothiazolinone is 1000mg/L. The stock solution shall be stored in the refrigerator for cold storage.

5.2.2 Drawing of the standard working curve

Accurately and respectively absorb the standard working solution (3.5) for 10µL; inject them into the liquid chromatograph in the order of the concentration from the dilute to the concentrated; perform the determination according to the chromatographic condition (5.2.1); use the peak area of the chromatographic peak as the ordinate AND use the corresponding concentration as the abscissa to draw the diagram; draw the standard working curve. The liquid chromatogram of the standard substance of methylisothiazolinone and chloromethylisothiazolinone is shown in Appendix A.

5.2.3 Sample Determination

Use micro syringes to accurately absorb 10µL of sample solution (5.1); inject into the liquid chromatograph; perform the determination according to the chromatographic condition (5.2.1); record the retention time and peak area of the chromatographic peak. From the peak area of the chromatographic peak, it can obtain the concentration of the corresponding tested substance from the standard curve. In the sample solution, both of the response value of methylisothiazolinone and chloromethylisothiazolinone shall be within the linear range of instrumental determination. For the samples in which the content of tested substance is high, it can take appropriate sample solution to perform determination after diluting with methanol.

5.3 Blank test

In addition to not-weigh the sample, it shall be performed according to the above procedure.

6 Results calculation

The results shall be calculated as formula (1).

Where.

W - the content of methylisothiazolinone or chloromethylisothiazolinone in sample, %;

c - the concentration of methylisothiazolinone or chloromethylisothiazolinone in the sample solution, which is obtained in the standard working curve. The unit is milligram per liter (mg/L);

V - final constant volume of the sample solution. The unit is liter (L);

m - the mass of the sample. The unit is gram (g);

The calculation results shall be accurate to 0.001.

......

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 8 pages — is available in the English PDF.

How to Download SN/T 1204-2003

1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.