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SN/T 1149-2019Quarantine identification method for coconut spleen (English PDF)

椰子缢胸叶甲检疫鉴定方法

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Issued by

General Administration of Customs

Level / Type

National · Recommended

Issue date

September 3, 2019

Implementation date

March 1, 2020

Scope

SN/T 1149-2019 is the English-translated version of 椰子缢胸叶甲检疫鉴定方法.

SN/T 1149-2019 — This standard applies to the quarantine and identification of coconut palm chestnut leaflet from imported palm plant seedlings, coconut fruits, and coconut fiber.

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Document preview — SN/T 1149-2019

National Standard of the People's Republic of China

Classification: B 16
Replacing: SN/T 1149-2002

Issued by: General Administration of Customs

Foreword
1 Scope
2 Terms and definitions
4 Method principle
5 Equipment and reagents
5.1 Equipment
5.2 Reagents
6 Detection and feeding
6.1 Detection of Palmae Seedlings
6.2 Detection of coconut fruit and coconut shell fiber
6.3 Feeding
7 Laboratory identification
7.1 Preparation
7.2 Morphological identification
8 Result judgment
9 Specimen preservation

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

This standard replaces SN/T 1149-2002 "Plant Quarantine Coconut Thorn Breast A quarantine identification method" and is in line with SN/T 1149-2002 the main technical changes are as follows.

--- The original standard title and structure were appropriately adjusted to make it more reasonable.

--- Added content such as synonyms, specimen preservation, and references to make the standard more complete.

--- Reorganized the description of the morphological characteristics of the adult Coconut pupa, so that its content is more substantial and the description is more detailed.

--- Removed spot inspection methods and sampling numbers for quarantine.

--- Adjusted Appendix A of the original standard to Appendix D, added the host, distribution, hazard and biological characteristics of Coconut Breastplate A this standard is proposed and managed by the General Administration of Customs of the People's Republic of China.

This standard was drafted. Shenzhen Customs of the People's Republic of China, Shenzhen Academy of Inspection and Quarantine.

1 Scope

This standard applies to the quarantine and identification of coconut palm chestnut leaflet from imported palm plant seedlings, coconut fruits, and coconut fiber.

2 Terms and definitions

The following terms and definitions apply to this document.

Measure the widest part of the body.

Refers to the longitudinal grooves on the back of the elytra, including rows of engraved dots.

The area between every two adjacent line patterns on the elytra is interline.

The small shield is a small bone piece at the rear of the mid-thoracic dorsal plate, located between the center of the base of the front pectoral dorsal plate and the inner angles of the two coleoptera.

The full-open type refers to the two claws being flattened, and the angle formed by the bases of the two is very large, generally more than 120 °.

3 Coconut thymus leaf methyl Taxonomic status. Coleoptera Coleoptera, Chrysomelidea of the family Ceratophylla, Hispiedae of the family Armoridae, Anisoderinee of the subfamily Subfamily, SN/T 1141-2019 ????? ????, ????,?

Way of transmission. It can be transmitted by long-distance transportation with coconut and other palm plants, fruits, fibers and other carriers, and it can also spread with wind.

For other related information, see Appendix A and Appendix B.

4 Method principle

According to the biological characteristics of Coconut scapula, collect the complete or shaped insect bodies from the incoming plants and plant products, packaging and transportation vehicles.

Specimens with intact morphological characteristics. According to the morphological characteristics of the Coconut pheasant, the identified parts including the head, pronotum Plates, elytra, abdomen, feet and other parts are determined based on morphological characteristics.

5 Equipment and reagents

5.1 Equipment

Stereo microscope,, micrometer, magnifying glass, scissors, tweezers, insect dissection needle, small writing brush, petri dish, finger tube, glass slide, cover Slides, alcohol lamps, beakers, slide labels, drying boxes, incubators.

5.2 Reagents

Ethanol, larval preservation solution (8% formalin).

6 Detection and feeding

6.1 Detection of Palmae Seedlings

When selecting points to observe the leaves, look at them from bottom to top, especially the old leaves in the lower part. Pay special attention to it first to see if there are adult insects on the leaf surface, and then Observe that each leaflet has an ovoid sac-like bulge of 2mm to 3mm, find the cysts, and cut the whole leaf. Put the leaf in the sample bag, and the adult Use finger tubes to hold larvae or pupae with 8% formalin vacuole and bring them back to the room for further inspection.

6.2 Detection of coconut fruit and coconut shell fiber

When selecting points for inspection, first observe whether the outer package has worms, then open the package, observe them fruit by fruit, pay attention to the pedicles or cracks that can conceal the insects, and Invert the bag to see the debris at the bottom of the bag. Samples of coconut fruit or coconut shell fibers with suspected worms may be taken for further laboratory inspections, and pay attention to Inspection of loading containers or loading containers.

6.3 Feeding

The immature worms, such as the pupa, larvae, and pupae of the coconut pupa, which need to be raised, together with the relevant parts of the host, should be at a temperature of 25 °C ~ 30 °C Humidity was observed at 70%, and identification was performed after emergence of adults.

7 Laboratory identification

7.1 Preparation

The surface of the insect body used for observation must be clean. If there is dirt or dust on the body surface, the specimen should be immersed in 70% ethanol and washed with a brush.

clean.

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7.2 Morphological identification

7.2.1 Main characteristics of adults

The main characteristics of the adults of the Trichodidae family are as follows.

--- Section 4, section 4-5, generally heal completely, sometimes leaving traces of section;

--- The head is posterior, the mouth is in the rear position, but the back is not visible;

--- The two antennae are very close to each other and sometimes almost connected;

--- The viscous hairs of the 1st to 3rd sections are single-forked.

7.2.2 Main characteristics of Submeridae

The main characteristics of the subfamily are as follows.

--- The body is generally long, without spines on the back, and the elytra sometimes has a ridge line;

--- The head is inserted into the chest cavity shallowly, all the mouth organs are exposed, the edge of the mouth is almost round, and the length is equal to or wider than the length;

--- The anterior lip base is obvious, and the hindfoot femur extends beyond the lateral margin of the coleoptera;

--- Fully open claw;

--- no egg sheath;

--- Larvae are parasitic, without tail.

7.2.3 Main characteristics of the adult

The main characteristics of the pupa breast armor adult are as follows.

--- The two sides of the body are nearly parallel, the front chest has no obvious side, curled back and forth, the width is equal, and the middle is convex;

--- Elytra has no obvious ridges, no extra engraved shield lines, and the hind leg segments are quite long.

7.2.4 Main characteristics of genus Breastplate adults (see Appendix B, C)

The main characteristics of the genus Breastplate are as follows.

--- 11 antennae;

--- The length of the elytra is about three times the sum of the head and chest length, and there is almost no complete coat.

7.2.5 Main characteristics of Coconut pheasant (see Appendix D)

7.2.5.1 Adult

The main characteristics of Coconut Tadpole Beetle Adults are as follows.

--- Body length is 7.5mm ~ 10mm, body width is 1.6mm ~ 2.0mm, reddish brown. Head protrudes forward, eyes are large, oval, the small eyes are fine, the frontal tumors are slightly raised, and smooth; the antennae are 11 knots, half of the elytra;

--- The pronotum is longer than wide, smooth and clean, with fine engraved points on the front, and the sides of the anterior part of the middle are slightly curled, the base is deep and the middle groove is deep, Arch backward

--- Small shield tongue-shaped;

--- Coleoptera is long, the female is slightly larger than the male, and its abdominal end is slightly larger than the front end, and the male abdominal end is equal to the end. The sides are nearly parallel, the engraving points are thick and neatly arranged, with 8 rows of lines, no ridges between rows, and no small shields.

--- Short forefoot, wide and flat footpads, fully open claws; thin and long hind legs, 1 triangular tooth process at 1/5 of the medial proximal end, tibial base the part is concave and concave, corresponding to the leg joint teeth.

SN/T 1141-2019 ????? ????, ????,?

7.2.5.2 Eggs

The main characteristics of Coconut pheasant's thorax eggs are as follows.

--- Egg length is 1.5mm and width is 1.0mm;

--- Oval shape, shaped like watermelon seeds, tan.

7.2.5.3 Old mature larva

The main characteristics of the mature larvae of the Coconut pheasant are.

--- Average body length is 9.5mm, head width is 1.5mm, semi-cylindrical, without feet;

--- Flat dorsal and ventral surface, cream-colored; pronotum brown, triangles of ossification;

--- Abdominal dorsal and ventral surface is slightly raised, dorsal and ventral surface is shiny, 11 sections of body, 6 pairs of fine hairs on each side of each section.

7.2.5.4

The main characteristics of Coconut sternum leaf chestnuts are as follows.

--- Body length is 7mm ~ 8mm, body width is 1.6mm, orange to yellow-brown;

--- Abdominal dorsal and ventral surface is slightly raised, able to move freely; hairy, eyes are black, palate is tan.

8 Result judgment

Based on the morphological characteristics of adults, the characteristics of eggs, larvae, and pupae were used as references, and it was judged to be 7.2.5.1 for Coconut pupae.

9 Specimen preservation

Coconut sturgeon leaf beetle specimens can be stored in a sample bottle containing 75% ethanol, and the Chinese name, scientific name, collection person, collection Time, quarantine and place of origin, samples (specimens) should be kept for at least 6 months.

SN/T 1141-2019 ????? ????, ????,?

Appendix A

(Informative appendix) Coconut Wing Breast Leaf Methyl A. 1 host Coconut, yata coconut, oil palm, king palm, betel palm, thorn sunflower and sago coconut.

A. 2 Geographical distribution Distributed in Malaysia, Singapore, Brunei, Philippines, Indonesia, Sri Lanka.

A. 3 Harm

Adults feed on the leaves; larvae enter from the surface and eat on the leaves. Each larva eats food towards the leaf end throughout its life, leaving only the sacral part Upper surface, and quickly dry brown. There are often multiple worms on the leaves that harm simultaneously. The victim leaves are curled, scorched, and easily broken by wind. Much more this fungus invades and produces silver-gray fruiting bodies. When seriously injured, the adult and larva together can reduce the photosynthetic leaf area by 75%, so that the fruit falls, Yield loss can reach 80%; the tree crown is brownish gray when viewed from a height, and the landscape is greatly affected.

A. 4 Biological characteristics Adults often endanger older leaves (ie, fourth to fifth leaves) of the lower part of the plant, but when the damage is severe, the young leaves are also damaged. Adults first A small hole is bitten on the back of the leaf to lay a single egg. Then, the feces are covered and the glue is secreted to form an oval with a length of about 2mm to 3mm.

Capsule-shaped bulging. The newly hatched larvae drilled directly into the leaf and ate towards the end of the leaf, forming a pupal with a width of about 1 cm and a length of 10 to 20 cm.

Road, emptying the mesophyll leaves only the epidermis, the surface is bubbly. Old mature larvae pupate in the tunnel.

SN/T 1141-2019 ????? ????, ????,?

Appendix B

(Informative appendix) Number Scientific Name Distribution Host Fiji, Philippines, Samoa, Solomon Islands, Tonga SN/T 1141-2019 ????? ????, ????,?

Table B. 1 (continued)

Number Scientific Name Distribution Host SN/T 1141-2019 ????? ????, ????,?

Appendix C

(Informative appendix) SN/T 1141-2019 ????? ????, ????,?

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(Quoted from Staines, 2015) SN/T 1141-2019 ????? ????, ????,?

Appendix D

(Informative appendix) Morphological characteristics of Coconut pheasant

Figure D. A. Rear view of adult D. B. Back view of larva 3 dorsal view

SN/T 1141-2019 ????? ????, ????,?

references

[1] Chen Naizhong. Compilation of Incoming Plant Quarantine Pests in China [M]. China Agricultural Publishing House, 1996, 264-266.

[2] Chen Naizhong, An Yulin, Zhang Runzhi, et al. Incoming plant quarantine pests in China --- insect rolls [M]. China Agricultural Publishing Press, 2009, 250-252.

[3] Chen Shizhen, et al. Zoozoe of China. Ironidae [M]. China Agricultural Publishing House, 1986, 1-189.

[4] Geng Bingjin. Atlas of Plant Quarantine Pests in China [M]. China Agricultural Press..1999, 100-111.

[5] Zhang Jun. Color map of plant quarantine pests [M]. Science Press..1993, 110-111.

[6] AldabaVC. Thecoconutleaf-mininistafionioflagona, Batasgas, andTayababas [J].

Philipp. J. Agric. , 1930, 1 (2).

CoconutPlantersRev. , 1972, 6 (4). 152-156.

[8] Gressit JL. Papuan-westpointHispineline Beetles [J]. Pacif. Ins. , 1960, 2 (1). 1-84.

[9] HespenheideH A. Bionomics-miningsects [J]. AnnualReevelifeEntomology, 1991, 36. 535-560.

1913, 8 (2). 127-134.

[11] LeveRJaw. AnycoconutingSingapore [J]. Malayan Agricultural Journal, 1951, 35. 79-82.

[12] Staines CL. Catalofofhisenesofowed (Coleoptera. Chrysomidee. Cassine).

2015. http. // entomolog. si. edu/Collections_Coloprepera-Hispines. html SN/T 1141-2019 ????? ????, ????,?

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