SN/T 0764-2011Quarantine protocol for newcastle disease (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

Instead of the standard SN/T 0764-1999 "export poultry disease virus testing methods", SN/T 1686-2005 "Newcastle disease virus Virulent strains fluorescent RT-PCR detection method ", SN/T 1109-2002" Newcastle micro hemagglutination inhibition test procedures "and SN/T 1110-2002 "Newcastle disease virus isolation and identification methods." this standard compared with SN/T 0764-1999, SN/T 1109-2002, SN/T 1110-2002 and SN/T 1686-2005, the main to technical changes are as follows.

--- Increased clinical diagnosis;

--- Increased reverse transcription - polymerase chain reaction (RT-PCR);

--- Remove the chicken average death time (MDT) Virulence and intravenous pathogenicity index (IVPI).

This revised standard adopts OIE "Terrestrial animal diagnostic tests and vaccines Handbook" (2009 Edition) (ManualofDiagnosticTestsand

2.3.14 Chapter VaccinesforTerrestrialAnimals) in.

This compared with the OIE standard 2.3.14 chapter, the main technical differences are as follows.

--- This standard is fully adopted Isolation and Identification, hemagglutination and hemagglutination inhibition test method OIE provisions;

--- This increases the OIE standards mentioned but no detailed rules reverse transcription - polymerase chain reaction, fluorescence reverse transcription - polymerase the specific test step chain reaction;

--- This standard deleted OIE mentioned but not detailed the steps of enzyme-linked immunosorbent assay.

Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.

This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Beijing Entry-Exit Inspection and Quarantine of Jiangsu People's Republic of China, People's Republic of China Shandong Entry-Exit Inspection and Quarantine of Guangdong People's Republic of China, People's Republic of China Entry-Exit Inspection and Inner Mongolia Quarantine Bureau.

1 Scope

This standard specifies the clinical diagnosis of avian Newcastle disease, virus isolation and identification, hemagglutination and hemagglutination inhibition test, reverse transcription - polymerase chain reaction should, fluorescence reverse transcription - polymerase chain reaction procedures.

This standard applies to the import and export of poultry and its products in Newcastle disease quarantine.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

Laboratory use specifications and test methods GB/T 6682 Analysis

3 Clinical diagnosis

4 Laboratory diagnosis