Valid

GB/Z 42840-2023Nanotechnologies - Determination of silver nanoparticles potency by release of muramic acid from Staphylococcus aureus (English PDF)

纳米技术利用金黄色葡萄球菌释放的胞壁酸检测银纳米颗粒的效力

Preview PDF

This is a limited preview

Buy now to download the full PDF (30 pages)

Need quotation for your management?

Get a formal quotation for GB/Z 42840-2023 in 30 minutes.

Issued by

State Administration for Market Regulation, China National Standardization Administration

Level / Type

National · Recommended

Issue date

August 6, 2023

Implementation date

August 6, 2023

Scope

GB/Z 42840-2023 is the English-translated version of 纳米技术利用金黄色葡萄球菌释放的胞壁酸检测银纳米颗粒的效力.

GB/Z 42840-2023 is the Chinese standard "Nanotechnologies - Determination of silver nanoparticles potency by release of muramic acid from Staphylococcus aureus".

Its scope clause reads: This paper describes a method using gas chromatography-mass spectrometry (GC-MS) to quantitatively evaluate the effects of silver nanoparticles on Staphylococcus aureus cells.

Methods for wall degradation and muramic acid release efficacy. Its clauses include normative reference documents; terms and definitions.

Found what you need?

Document preview — GB/Z 42840-2023

National Standard of the People's Republic of China

ICS: 07.030
Classification: A 43

Issued by: State Administration for Market Regulation, China National Standardization Administration

Preface
Introduction
1 Scope
2 Normative reference documents
3 Terms and Definitions
4 Symbols and abbreviations
5 Principle
6 Sample preparation for MA detection
6.1 Bacterial growth and AgNP treatment
6.2 Cell wall hydrolysis
6.3 Cell wall derivatization
6.4 Gas Chromatography-Mass Spectrometry
7 Data analysis and interpretation of results
8 Detection methods for silver nanoparticle samples
Appendix A (Informative) Gas Chromatography-Mass Spectrometry
Appendix B (Informative) Internal Standard
Appendix C (informative) Laboratory preparation method of silver nanoparticle reference material
Appendix D (informative) Preparation of silver nanoparticle suspension

1 Scope

This paper describes a method using gas chromatography-mass spectrometry (GC-MS) to quantitatively evaluate the effects of silver nanoparticles on Staphylococcus aureus cells.

Methods for wall degradation and muramic acid release efficacy.

2 Normative reference documents

This document has no normative references.

3 Terms and definitions

The following terms and definitions apply to this document.

3.1

The property of a compound or substance to kill bacteria (bactericidal) or inhibit bacterial growth (bacteriostatic).

3.2

A physical technique that can be used to detect the particle size distribution of small particles in suspension or polymers in solution.

3.3 gas chromatography-mass spectrometry gaschromatography-massspectrometry; GC-MS

A technique that combines gas chromatography and mass spectrometry for qualitative and quantitative analysis of volatile compounds in samples to be tested (see Appendix A).

3.4 Grampositivebacteria

In Gram staining, bacteria that can be stained/not destained by organic solvents.

3.5 internal

A compound of known concentration added to a sample to aid in the qualitative identification and/or quantitative determination of the sample's components (see Appendix B).

[Source. ISO 20752.2014,3.2]

3.6 Limit of detection limitofdetection;LOD

The analyte in the test sample can be reliably detected but is not required to be quantified, as demonstrated by collaborative testing or other appropriate verification.

Minimum amount or concentration.

[Source. ISO 8196-1.2009,3.4.3]

GB/T 37254 | DZ/T 0175 | GB/T 8170 | GB/T 43598 | GB/T 41067 | […]

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 30 pages — is available in the English PDF.

How to Download GB/Z 42840-2023

1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.