GB/T 5009.116-2003Determination of oxytetracyline，tetracyline and chlortetracycline residues in meat(HPLC) (English PDF)

Foreword

This Standard replaces GB/T 14931.1-1994 "Method for determination of oxytetracyline, tetracyline and chlortetracycline residues in meat (HPLC)".

Compared to GB/T 14931.1-1994, the main changes of this Standard are as follows.

- MODIFY the Chinese name of the standard; the Chinese name of the standard is changed to "Determination of oxytetracyline, tetracyline and chlortetracycline residues in meat (HPLC)";

- MODIFY the structure of the original standard according to GB/T 20001.4-2001 "Rules for drafting standards - Part 4: Methods of chemical analysis".

1 Scope

This Standard specifies the determination method of oxytetracyline, tetracyline and chlortetracycline residues in meat.

This Standard applies to the determination of oxytetracyline, tetracyline and chlortetracycline residues in all kinds of livestock and poultry meat.

In this Standard, the detection limit is 0.15 mg/kg for oxytetracycline, 0.20 mg/kg for tetracyline and 0.65 mg/kg for chlortetracycline.

2 Principle

After being extracted and filtered through the microporous membrane, the sample is directly injected, separated by reversed-phase chromatography, detected by UV detector and compared with the standard; the appearance order of the peak is oxytetracyline, tetracyline and chlortetracycline. QUANTIFY by standard addition method.

3 Reagents

3.1 Acetonitrile (analytical reagent).

3.2 0.01 mol/L uranium dihydrogen phosphate solution. WEIGH 1.56 g (accurate to ± 0.01 g) of sodium dihydrogen phosphate (NaH2PH4 · 2H2O); DISSOLVE in distilled water to 100 mL; FILTER through a microporous membrane (0.45 µm) for further use.

3.3 Oxytetracyline (OTC) standard solution. WEIGH 0.0100 g (accurate to ± 0.0001 g) of oxytetracyline; DISSOLVE with 0.1 mol/L hydrochloric acid solution to 10.00 mL; the solution contains 1 mg of oxytetracyline per milliliter.

3.4 Tetracyline (TC) standard solution. WEIGH 0.0100 g (accurate to ± 0.001 g) of tetracyline; DISSOLVE with 0.01 mol/L hydrochloric acid solution to 10.00 mL; the solution contains 1 mg of tetracyline per milliliter.

3.5 Chlortetracycline (CTC) standard solution. WEIGH 0.0100 g (accurate to ± 0.0001 g) of chlortetracycline; DISSOLVE in distilled water to 10.00 mL; the solution contains. […]