GB/T 47399-2026Test method for the antibacterial activity of sanitary ware - Luminescence method (English PDF)
卫生陶瓷抗菌性能试验方法 荧光法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
March 31, 2026
Implementation date
October 1, 2026
Scope
GB/T 47399-2026 is the English-translated version of 卫生陶瓷抗菌性能试验方法 荧光法.
GB/T 47399-2026 is the Chinese standard "Test method for the antibacterial activity of sanitary ware - Luminescence method".
Its scope clause reads: This document describes the principle, general requirements, reagents and culture media, and setup for testing the antibacterial properties of antibacterial sanitary ceramics and their products using a fluorescence method.
Preparation and materials, test strains, samples and pretreatment, testing procedures, result calculation, test reports, etc. This document applies to the testing of the antibacterial properties of antibacterial sanitary ceramics and their products, as well as antibacterial sanitary ceramics modified with metal ions and rare earth elements. Its clauses include terms and definitions; principles.
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Document preview — GB/T 47399-2026
National Standard of the People's Republic of China
- ICS
- 91.140.70
- Classification
- Q 31
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 3.
1 Scope
This document describes the principle, general requirements, reagents and culture media, and setup for testing the antibacterial properties of antibacterial sanitary ceramics and their products using a fluorescence method.
Preparation and materials, test strains, samples and pretreatment, testing procedures, result calculation, test reports, etc.
This document applies to the testing of the antibacterial properties of antibacterial sanitary ceramics and their products, as well as antibacterial sanitary ceramics modified with metal ions and rare earth elements.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB 19489-2008 General Requirements for Laboratory Biosafety
SN/T 5432-2023 Determination of antibacterial properties of antibacterial textiles - ATP fluorescence analysis method
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
The product has the ability to kill and inhibit the growth and reproduction of bacteria.
3.2 Antibacterial ceramic
Ceramic products with antibacterial properties on the surface.
3.3 control sample
Ceramic samples or silicate glass plates used to verify the growth conditions of test bacteria, without the addition of any antibacterial ingredients.
3.4 fluorescence method
The fluorescence intensity (IB) of viable bacterial cells in the sample solution was determined using an adenosine triphosphate (ATP) fluorometer, and the result was calculated based on the logarithmic value of the viable bacterial content.
The method for calculating the corresponding viable bacterial content (CB) from the lgCB-logarithmic fluorescence intensity standard curve is as follows.
4 Principles
Bacteria were quantitatively inoculated onto the antibacterial sanitary ceramic samples to be tested and the control samples, and a contact culture method involving surface coating and sealing in petri dishes was used.
The formula was used to perform immediate elution and elution after incubation, respectively. The fluorescence intensity (IB) of the residual viable bacteria in the recovered solution was measured using a fluorescence method, and the result was calculated according to the formula.
The antibacterial rate or antibacterial activity value is calculated to evaluate the antibacterial performance of sanitary ceramic samples.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 44 pages — is available in the English PDF.
Referenced standards
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