GB/T 47062-2026Animal and vegetable fats and oils - Determination of hydroxytyrosol and tyrosol content in extra virgin olive oils - Reverse phase high performance liquid chromatography method (English PDF)

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents".

This document adopts ISO 23942:2022 "Determination of Hydroxytyrosol and Tyrosol Content in Extra Virgin Olive Oil by Reversed-Phase High Performance Liquid Chromatography" as equivalent to standard ISO 23942:2022. Chromatography (RP-HPLC)

The following minimal editorial changes have been made to this document.

---To align with existing standards, the standard name has been changed to "Content of Hydroxytyrosol and Tyrosol in Extra Virgin Olive Oil from Animal and Vegetable Oils". Determination by reversed-phase high-performance liquid chromatography.

1 Scope

This document describes the determination of hydroxytyrosol and tyrosol content in extra virgin olive oil using reversed-phase high-performance liquid chromatography-spectroscopy. method.

This method is applicable to the determination of hydroxytyrosol and tyrosol content in all commercial olive oils.

2 Normative references

There are no normative references in this document.

3 Terms and Definitions

The following terms and definitions apply to this document.

The URLs for the terminology databases maintained by ISO and IEC for standardization are as follows. […]

5 Reagents

5.1 Phosphoric acid. 85% by volume.

5.2 Methanol. chromatographic grade.

5.3 Acetonitrile. chromatographic grade.

5.4 Water. chromatographic grade.

5.5 Ethanol. 96% by volume.

5.6 Sulfuric acid. 96% by volume.

5.7 Methanol/water solution. volume ratio 80.20.

5.8 Standard. Hydroxytyrosol or 3,4-dihydroxyphenylethanol (C8H10O3, CAS. 10597-60-1), purity >=98%.