GB/T 46927-2025Spatio-temporal omics - Specification for dataset format (English PDF)

3.1 Spatio-temporal omics

Building upon spatial transcriptomics, this extends to multiple omics levels, including genomics, epigenomics, and proteomics, utilizing spatial analysis...

Multi-omics technologies can be used to perform molecular localization of tissues or organisms at single-cell or subcellular resolution, combined with time-series sampling or computation.

It can be inferred to achieve spatiotemporal dynamic analysis of biological processes such as development, physiology, and pathology.

Note. Spatial transcriptomics refers to the use of high-throughput technologies to locate transcripts at single-cell or subcellular resolution within the spatial context of tissues or organs.

This enables space observation at multiple scales, including molecules, cells, tissues, and organs.

3.2

The smallest spatial unit capable of capturing biomolecules.

Note. The spatial location of biomolecules captured within this unit region cannot be distinguished.

3.3 spatio-temporal chip

A specialized chip that arranges biomolecule capture units in a specific pattern to capture nucleic acid fragments in space.

Note. All biomolecule capture units on the chip are distinguished by CID, and all captured nucleic acid sequences on the chip are distinguished by MID. CID, MID, and nucleic acid...

Sequence information was obtained using high-throughput sequencing. The arrangement of the biomolecule capture units in the spatiotemporal chip is not limited; it can be arranged in a square or other configurations.

They are arranged in an equilateral triangle, with a typical spacing of 500nm to 100µm.

3.4 Serial barcode

In biological fields such as high-throughput sequencing and cell analysis, short nucleic acid sequences are used to label different samples, cells, or nucleic acid molecules.

Label.