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GB/T 46540-2025Textiles - Determination of microorganisms (English PDF)

纺织品　微生物的测定

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

October 31, 2025

Implementation date

May 1, 2026

Scope

GB/T 46540-2025 is the English-translated version of 纺织品　微生物的测定.

GB/T 46540-2025 is the Chinese standard "Textiles - Determination of microorganisms". Its scope clause reads: This document describes the sample collection and pretreatment for microbial assays in textiles, as well as the determination of total bacterial count, coliforms, and aureus in textiles.

Methods for the determination of Staphylococcus, Candida albicans, Pseudomonas aeruginosa, and beta-hemolytic streptococci. This document applies to all types of textiles. Its clauses include terms and definitions.

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Document preview — GB/T 46540-2025

National Standard of the People's Republic of China

ICS: 59.080.01
Classification: W 04

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Preface
1.Scope
2 Normative References
3.Terms and Definitions
4.Sample Collection and Processing
5.Determination of total bacterial count
6.Determination of coliform bacteria
7.Determination of Staphylococcus aureus
8.Determination of Candida albicans
9.Determination of Pseudomonas aeruginosa
10.Determination of Group A beta-hemolytic streptococci
Appendix A (Normative) Culture Media and Reagents
Appendix B (Normative) LAMP Primers
Appendix C (Normative) Primers and Probes for Real-Time Fluorescent PCR
Appendix D (Informative) Graphs for Determining Positive Results in LAMP and PCR Tests

1 Scope

This document describes the sample collection and pretreatment for microbial assays in textiles, as well as the determination of total bacterial count, coliforms, and aureus in textiles.

Methods for the determination of Staphylococcus, Candida albicans, Pseudomonas aeruginosa, and beta-hemolytic streptococci.

This document applies to all types of textiles.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.

For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.

This document.

GB/T 8170 Rules for rounding off numerical values and the representation and determination of limiting values

GB 19489 General Requirements for Laboratory Biosafety

GB/T 36433-2018 Textiles - Quantitative Analysis of DNA from Mixtures of Cashmere and Sheep Wool - Fluorescent PCR Method

3 Terms and Definitions

The terms and definitions defined in GB/T 36433-2018, as well as the following terms and definitions, apply to this document.

3.1 Colony-forming units (CFU)

The unit for determining the concentration of viable bacteria in a sample is based on the number of colonies formed on a solid culture medium.

3.2

Under an isothermal environment of 60°C~65°C, DNA is in a dynamic equilibrium state. At this temperature, four specific primers are used with a high-performance...

Active chain replacement DNA polymerase is a technology that enables DNA to undergo nucleic acid amplification in a short time.

Note. The reaction produces a large amount of amplification products, namely magnesium pyrophosphate, which is a white precipitate. The presence or absence of the white precipitate can be determined by visually observing it.

3.3 Real-time fluorescence quantitative PCR; PCR

A fluorescent group is added to the PCR reaction system, and the accumulation of fluorescence signal is used to monitor the entire PCR process in real time. Then, the fluorescence signal is used to analyze the results of the unprocessed PCR.

DNA amplification methods that use known templates for quantitative or qualitative analysis.

[Source. GB/T 36433-2018, 3.2]

3.4 Ct value cyclethreshold

The number of cycles required for the fluorescence signal in each reaction tube to reach the set threshold.

[Source. GB/T 36433-2018, 3.5]

GB/T 45365 | GB/T 45367 | GB/T 45370 | GB/T 46542 | GB/T 46539 | […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 44 pages — is available in the English PDF.

Referenced standards

Normative references

Editions of GB/T 46540

Edition	Title	Revision	Status
GB/T 46540-2025	Textiles - Determination of microorganisms	current edition	Current

This page sells the current edition, GB/T 46540-2025. Earlier editions are listed for reference only.

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