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GB/T 45774.1-2025Quantitative determination of antibacterial activity of ceramic tile surfaces - Test methods - Part 1: Ceramic tile surfaces with incorporated antibacterial agents (English PDF)

陶瓷砖表面抗菌活性的定量测定 试验方法 第1部分:含有抗菌剂陶瓷砖表面

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Issued by

State Administration for Market Regulation, China National Standardization Administration

Level / Type

National · Recommended

Issue date

June 30, 2025

Implementation date

January 1, 2026

Scope

GB/T 45774.1-2025 is the English-translated version of 陶瓷砖表面抗菌活性的定量测定 试验方法 第1部分:含有抗菌剂陶瓷砖表面.

GB/T 45774.1-2025 — This document describes a test method for evaluating the antimicrobial activity of antimicrobial agents applied to the surfaces of glazed and unglazed ceramic tiles.

This document does not cover secondary effects on the tile surface due to the antimicrobial treatment, such as chemical resistance, stain resistance or small colour variations.

For chemical resistance, see ISO 10545-13; for stain resistance, see ISO 10545-14; for small color differences, see ISO 10545-16. The test results obtained by this document are only applicable to this document and the test conditions specified in it.

The antibacterial activity under different conditions cannot reflect the activity under various factors such as temperature, humidity, different bacterial species, and nutritional conditions.

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Document preview — GB/T 45774.1-2025

National Standard of the People's Republic of China

ICS
91.100.25
Classification
Q 31

Issued by: State Administration for Market Regulation, China National Standardization Administration

Contents

  • Preface
  • Introduction
  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 4 Ingredients
  • 4.1 Strain
  • 4.2 Culture media and reagents
  • 4.2.1 Nonionic surfactants
  • 4.2.2 Nutrient broth
  • 4.2.3 1/500 Nutrient Broth
  • 4.2.4 Nutrient agar medium
  • 4.2.5 Soybean Casein Digest Broth with Lecithin and Polysorbate 80 (SCDLP)
  • 4.2.6 Phosphate Buffered Saline (PBS)
  • 5 Sample
  • 5.1 Size
  • 5.2 Control Sample
  • 5.3 Preprocessing
  • 5.4 Number of samples.
  • 6 Step
  • 6.1 Sample inoculation preparation
  • 6.2 Covering film
  • 6.3 Sample inoculation
  • 6.4 Recovery of bacteria from the blank control sample at t = 0 h
  • 6.5 Cultivation Period
  • 6.6 Recovery of bacteria from samples after culture
  • 6.7 Count of viable bacteria eluted from sample
  • 7 Verification and Calculation
  • 7.1 General requirements
  • 7.2 Evaluation criteria for effective testing
  • 7.3 Calculation of antimicrobial activity
  • 8 Test Report
  • References

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

This document is Part 1 of GB/T 45774 "Test method for quantitative determination of antimicrobial activity on ceramic tile surfaces".

The following parts have been published.

--- Part 1: Surfaces of ceramic tiles containing antimicrobial agents;

--- Part 2: Surface of ceramic tiles containing photocatalytic antimicrobial agents.

This document is equivalent to ISO 17721-1:2021 "Test method for quantitative determination of antimicrobial activity on ceramic tile surfaces - Part 1: Containing Antimicrobial Agents for Ceramic Tile Surfaces.

Introduction

The antibacterial activity of ceramic tiles is the number of viable cells found after inoculation and culture of bacteria in ceramic tiles with antibacterial properties and blank controls.

The logarithmic difference of the difference is used to characterize the antibacterial performance of ceramic tile products. Antibacterial property has become one of the important indicators for evaluating the performance of ceramic tiles.

The standardization work has attracted widespread attention at home and abroad. In this regard, the ISO 17721 series of standards developed by the International Organization for Standardization (ISO) is Method standards in the fields of manufacturing, consumer health and hygiene are divided into two categories. antibacterial ceramic tiles with antimicrobial agents and photocatalytic antibacterial ceramic tiles.

China has already carried out domestic conversion work for this series of standards.

GB/T 45774 "Test method for quantitative determination of antimicrobial activity on ceramic tile surfaces" is a method standard for the antimicrobial activity of ceramic tile surfaces.

This standard aims to describe the test method for the antibacterial activity of antimicrobial ceramic tiles and photocatalytic antibacterial ceramic tiles, and is intended to consist of two parts.

--- Part 1: Surface of ceramic tiles containing antimicrobial agents. The purpose is to describe the test method for the antimicrobial activity of antimicrobial ceramic tiles containing antimicrobial agents.

Inspectors provide guidance.

--- Part 2: Surface of ceramic tiles containing photocatalytic antibacterial agents. The purpose is to describe the antibacterial activity test of photocatalytic antibacterial ceramic tiles Provide guidance to testers.

Quantitative test for the antibacterial activity of ceramic tile surfaces Method Part 1: Surface treatment of ceramic tiles containing antimicrobial agents

1 Scope

This document describes a test method for evaluating the antimicrobial activity of antimicrobial agents applied to the surfaces of glazed and unglazed ceramic tiles.

This document does not cover secondary effects on the tile surface due to the antimicrobial treatment, such as chemical resistance, stain resistance or small colour variations.

For chemical resistance, see ISO 10545-13; for stain resistance, see ISO 10545-14; for small color differences, see ISO 10545-16.

The test results obtained by this document are only applicable to this document and the test conditions specified in it.

The antibacterial activity under different conditions cannot reflect the activity under various factors such as temperature, humidity, different bacterial species, and nutritional conditions.

2 Normative references

This document has no normative references.

3 Terms and Definitions

The following terms and definitions apply to this document.

antibacterial

Inhibition of bacterial growth on the surface of a product or description of the effect of an agent that inhibits bacterial growth on the surface of a product.

antibacterial agent

Drugs or compounds that can kill or inhibit the growth of bacteria on the surface of products through surface antimicrobial (3.1) treatment.

The logarithmic difference in viable cell counts found after inoculation and culture of bacteria between the antimicrobial-treated product and the untreated control.

4 Materials

4.1 Strains

This test method uses the following strains.

a) Escherichia coli;

b) Staphylococcus aureus.

The test method should be carried out using any of the above strains. The strains used in the test are shown in Table 1.Other strains can also be used with this test method.

For tests, the test report should provide detailed information on the bacterial strains used, culture conditions and test procedures.

The transfer and subculture of the strain should be carried out in a biosafety cabinet. Use a sterile inoculation loop to inoculate the strain into the nutrient agar medium.

After incubation at (37±1)°C for 18h~24h, store at 5°C~10°C. The culture temperature and time.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 12 pages — is available in the English PDF.

Referenced standards

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