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GB/T 44396-2024Chemicals—Rare minnow (Gobiocypris rarus) embryo acute toxicity test (English PDF)

化学品 稀有鮈鲫胚胎急性毒性试验

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

August 23, 2024

Implementation date

March 1, 2025

Scope

GB/T 44396-2024 is the English-translated version of 化学品 稀有鮈鲫胚胎急性毒性试验.

GB/T 44396-2024 is the Chinese national standard covering the acute toxicity test that exposes rare minnow embryos to a chemical in a 24-well plate — the information required on the test substance, the static, semi-static and flow-through ways of renewing the medium, the equipment and materials, the pre-test preparation and the test procedure, the quality control with its in-plate control wells that catch contamination coming from the plate itself, and the reporting of the median lethal concentration. Informative appendices carry the normal course of embryo development, the water quality conditions, reference figures for telling the eggs apart, and the layout of the 24-well plate. First edition, in force from 1 March 2025. Issued on 23 August 2024, it has been in force since 1 March 2025.

Document preview — GB/T 44396-2024

National Standard of the People's Republic of China

ICS
13.300
Classification
A 80

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • PrefaceIII
  • 1 Scope1
  • 2 Normative references1
  • 3 Terms and Definitions1
  • 4 Test substance information1
  • 5 Test Principles2
  • 6 Equipment and Materials2
  • 7 Preliminary preparation for the experiment3
  • 8 Test procedures4
  • 9 Quality Control6
  • 10 Data and Reports7
  • Appendix A (Informative) Normal development of rare crucian carp embryos8
  • Appendix B (Informative) Test water quality conditions9
  • Appendix C (Informative) Reference Figure for Identification of Fish Eggs in Acute Toxicity Test of Rare Gobius crucianus Embryos10
  • Appendix D (Informative) 24-well cell culture plate layout12
  • References13

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting is required.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the National Technical Committee on Hazardous Chemicals Management Standardization (SAC/TC 251).

This document was drafted by: Shanghai Testing Center, Solid Waste and Chemicals Management Technology Center of the Ministry of Ecology and Environment, and Aquatic Biology of the Chinese Academy of Sciences.

Biological Research Institute, Shenyang Chemical Testing Technology (Nantong) Co., Ltd., Ningbo Customs Technology Center, Suzhou Huace Safety Assessment Technology Service Co., Ltd., Guizhou Jianande Technology Co., Ltd., Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Microbiological Analysis and Testing Center).

The main drafters of this document are: Chen Xiaoqian, Yang Jing, Zhang Ying, Liu Yanan, Yin Haowen, Liu Hongying, Liu Xiaojian, Wang Jianwei, He Yongfeng, Li Zixuan, Liu Hanwei, Zhang Yuelong, Pi Tianxing, Tian Baolian, Yang Hongbo, Dong Ronggui, Wu Xinying.

Acute toxicity test of chemicals on embryos of Gobiocypris rare

1 Scope

This document describes the test method for acute toxicity of chemicals to rare crucian carp embryos, including the required information on the test substance, test principle, instrumentation, and preparation, pre-test preparation, test procedures, quality control, data and reporting.

This document is applicable to testing the acute toxic effects of chemicals on embryos of Gobiocypris rare.

2 Normative references

GB/T 27861

3 Terms and definitions

The terms and definitions defined in GB/T 27861 and the following apply to this document.

3.1 Static test

The test is conducted in a way that the test medium is not replaced or flows during the entire test process.

3.2 Semi-static test

A test conducted with the test medium replaced at regular intervals (e.g. 24 h).

3.3 Flow-through test

A test performed in such a way that the test medium is automatically and continuously replaced throughout the test.

3.4 median lethal concentration

The concentration of the test substance that causes mortality in 50% of the tested fish eggs. Note. Expressed as LC50.

3.5 Internal plate control

During the test, the 24-well cell culture plate was used to verify the potential contamination caused by the cell culture plate manufacturer or operator.

The 4 culture wells are designed to minimize risk and any plate effects (such as temperature gradients) that may affect the assay results.

4 Test substance information

The required information for the test substance includes.

a) CAS number;

b) structural formula;

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.

Referenced standards

Normative references

GB/T 27861

Editions of GB/T 44396

EditionTitleRevisionStatus
GB/T 44396-2024Chemicals - Rare minnow(Gobiocypris rarus) embryo acute toxicity testcurrent editionCurrent

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