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GB/T 43650-2024Technical procedures for DNA species identification of wild animals and their products (English PDF)

野生动物及其制品DNA物种鉴定技术规程

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

March 15, 2024

Implementation date

July 1, 2024

Scope

GB/T 43650-2024 is the English-translated version of 野生动物及其制品DNA物种鉴定技术规程.

GB/T 43650-2024 is the Chinese national standard covering technical procedures for DNA species identification of wild animals and their products. Issued on 15 March 2024, it has been in force since 1 July 2024.

Document preview — GB/T 43650-2024

National Standard of the People's Republic of China

ICS
65.020.01
Classification
B 60

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • PrefaceIII
  • 1 Scope1
  • 2 Normative references1
  • 3 Terms, definitions and abbreviations1
  • 3.1 Terms and Definitions1
  • 3.2 Abbreviations2
  • 4 Sample collection and storage2
  • 4.1 Sample Collection2
  • 4.2 Sampling2
  • 4.3 Sampling and storage2
  • 4.4 Transportation3
  • 5 Structure of DNA Identification Procedure4
  • 6 DNA Identification Procedure4
  • 6.1 Sample preparation4
  • 6.2 DNA extraction5
  • 6.3 DNA purification5
  • 6.4 DNA quality assessment5
  • 6.5 Detection methods6
  • 7 Results presentation10
  • 8 Tracing Methods1010
  • 9 Pollution Prevention and Treatment1010
  • 10 Laboratory Area Setup10
  • 11 Instrument and reagent requirements10
  • 12 Laboratory Cleaning1011
  • 13 Safety protection1011
  • 14 Waste Disposal1011
  • Appendix A (Informative) Electrophoresis Detection Procedure11
  • A.1 Conventional electrophoresis detection method11 A.2 Chip electrophoresis detection method12 A.3 Other electrophoresis detection methods1213
  • Appendix B (Informative) Universal Primer Sequence Information13
  • B.1 Universal primers for Cytb gene13
  • B.2 COI gene universal primer13
  • B.3 12SrRNA gene universal primer13
  • B.4 16SrRNA gene universal primer13
  • Appendix C (Informative) Universal Primer PCR Reaction System and Reaction Procedure15
  • C.1 Reaction system15
  • C.2 Reaction Procedure15
  • Appendix D (Informative) Primers, reaction procedures and reaction systems for specific PCR16
  • C.1 Primer specific to Saigatatarica16
  • C.2 Snow leopard (Pantherauncia) specific primer16
  • C.3 Pantherapardus specific primer17
  • C.4 Tiger (Pantheratigris) specific primer17
  • C.5 Black bear (Ursusthibetanus) specific primer17
  • Appendix E (Informative) qPCR Reaction System and Reaction Procedure18
  • E.1 Reaction system18
  • E.2 Reaction Procedure18
  • Appendix F (Informative) dPCR Reaction System and Reaction Procedure19
  • F.1 Reaction system19
  • F.2 Reaction Procedure19
  • References20

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document was proposed by the State Forestry and Grassland Administration.

This document is under the jurisdiction of the National Technical Committee for Standardization of Wildlife Conservation, Management and Commercial Utilization (SAC/TC369).

This document was drafted by: Northeast Forestry University, Northeast Forestry University Judicial Appraisal Institute, Shenzhen BGI Life Sciences Institute, China Customs Science and Technology Bureau Scientific and Technical Research Center, Heilongjiang Provincial Public Security Department, Harbin Inspection and Certification Group Co., Ltd., Shenzhen Huada Forensic Technology Co., Ltd., Guangdong Donghua University Forensic Evidence Judicial Appraisal Institute, Heilongjiang Wildlife Research Institute, South China Animal Species Environmental Damage Judicial Appraisal Center, Nanning Hai Customs Technology Center, Nanjing Police College, Hefei Customs Technology Center, Guangxi Zhuang Autonomous Region Public Security Department, and Dongying City Public Security Bureau.

The main drafters of this document are: Bai Suying, Ma Yue, Wang Zhen, Li Bo, Zhang Wei, Sun Lei, Xu Yanchun, Zhang Lifeng, Wang Jian, Liu Wei, Yang Tiantian, Lan Tianming, Li Xiaoping, Wei Jinpu, Guo Xiaosen, Li Ning, Jin Yu, Zhang Yu, Sun Hongyu, Ma Yunlong, Jin Xiangxiang, Liu Chuang, Liu Changjing, Hu Shijia, Li Yunfei, Zhang Xincheng, Tang Hongkai.

DNA species identification of wild animals and their products Technical regulations

1 Scope

This document specifies the sample collection and storage, DNA identification procedures for DNA species identification of wild animals and their products.

The main technical requirements include composition, DNA identification procedures, result presentation, traceability methods, pollution prevention and treatment, laboratory area settings, etc.

This document applies to DNA testing and identification of species from which wild animals and their products are derived.

2 Normative references

GB 19489

GB/T 19495.2

GB/T 34796

NY/T 541-2016

3 Terms, definitions and abbreviations

3.1 Terms and Definitions

The following terms and definitions apply to this document.

3.1.1 DNA barcode

A relatively short conserved DNA fragment of a selected standard region used for species identification.

3.1.2

Use DNA testing technology to determine the taxonomic status (family, genus, species) of wild animals and their products.

3.1.3 Sequence alignment

Using computer algorithms and programs, determine the similarity and even homology of two or more nucleotide sequences.

3.1.4 Primers

Two synthetic complementary oligonucleotide fragments, one is complementary to the DNA template strand at the 5' end of the target region; the other is complementary to the DNA template strand at the 3' end of the target region.

The DNA template strand is complementary.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 22 pages — is available in the English PDF.

Referenced standards

Editions of GB/T 43650

EditionTitleRevisionStatus
GB/T 43650-2024Technical procedures for DNA species identification of wild animals and their productscurrent editionCurrent

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