GB/T 43006-2023Leather and fur - Determination of degradability by micro-organisms (English PDF)
皮革和毛皮 微生物降解性的测定
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Issued by
State Administration for Market Regulation, China National Standardization Administration
Level / Type
National · Recommended
Issue date
September 7, 2023
Implementation date
April 1, 2024
Scope
GB/T 43006-2023 is the English-translated version of 皮革和毛皮 微生物降解性的测定.
GB/T 43006-2023 — This document describes a test method for the microbial degradability of leather and fur panels. This document is applicable to the determination of microbial degradability of various types of leather and fur panels.
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Document preview — GB/T 43006-2023
National Standard of the People's Republic of China
- ICS
- 59.140.30
- Classification
- Y 46
Issued by: State Administration for Market Regulation, China National Standardization Administration
Contents
- Preface
- 1 Scope
- 2 Normative reference documents
- 3 Terms and Definitions
- 4 Symbols and abbreviations 1
- 5 Principle
- 5.1 General
- 5.2 Method A. Titration method to evaluate microbial degradability
- 5.3 Method B. IR method to evaluate microbial degradability
- 6 Reagents and Materials
- 7 Instruments and equipment 2
- 8 Test step
- 8.1 Collection and preparation of inoculants 5
- 8.2 Preparation of test samples and reference materials 5
- 8.3 Test conditions and culture time
- 8.4 Test end judgment
- 9 Quantitative Analysis
- 9.1 Titration method to evaluate microbial degradability (Method A)
- 9.2 IR method to evaluate microbial degradability (Method B)
- 10 The result means
- 11 Reliability of results 10
- 12 Test Report
- Appendix A (informative) Structural changes of this document compared with ISO.20136.202011
- Appendix B (informative) Technical differences between this document and ISO.20136.2020 and their reasons
- Appendix C (informative) Determination of biodegradation rate and degradation rate
- Appendix D (informative) Quantitative determination of microbial degradability of leather 16
- Appendix E (informative) Comparison of the microbial degradability of type I collagen by inoculants from different sources 20
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.
This document is modified to adopt ISO.20136.2020 "Determination of microbial degradability of leather".
Compared with ISO.20136.2020, this document has many adjustments in structure. A comparison list of structural number changes between the two documents See Appendix A.
Compared with ISO.20136.2020, there are many technical differences between this document and the clauses involved in the outer margin of the page.
Single lines (|) are marked. A list of these technical differences and their causes is provided in Appendix B.
The following editorial changes have been made to this document.
---The name of the standard is modified to "Determination of Microbial Degradability of Leather and Fur";
---Change the unit of CO2 content from "ppm" to "mg/kg";
---Change "tannery wastewater" and "urban sewage" to "inoculant";
---Change the untitled bar in "6.2 Stock Solution" to column form;
---Deleted the second-level title of "6.3 Minimum Medium";
--- Clarified the meaning of y and x in the standard curve equation of the calibration system of Equipment B;
---Deleted the formula numbers for chemical reaction equations and the corresponding relationships between different quantities;
---Changed the letter codes in some formulas;
---Added informative appendix A and appendix B;
---Changed the upper temperature limit during the test process of Method B in Appendix B of ISO.20136.2020;
---Deleted "References".
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
1 Scope
This document describes a test method for the microbial degradability of leather and fur panels.
This document is applicable to the determination of microbial degradability of various types of leather and fur panels.
2 Normative reference documents
This document has no normative references.
3 Terms and definitions
The following terms and definitions apply to this document.
inoculum
Tannery wastewater containing activated sludge.
Note. If there is no tannery nearby, use urban sewage containing activated sludge from the urban sewage treatment plant as the inoculant.
4 Symbols and abbreviations
The following symbols and abbreviations apply to this document.
atm. standard atmospheric pressure, 101325Pa.
Ba(OH)2.barium hydroxide.
C. Carbon.
CO2.carbon dioxide.
GL14.Thread used with H-SAV29/32 Erlenmeyer flask (2000mL).
GL18.Thread used with H-SAVH40/45 Erlenmeyer flask (5000mL).
H-SAV29/32.The inner and outer diameters of the conical flask orifice are 29mm and 32mm respectively.
H-SAVH40/45.The inner diameter and outer diameter of the Erlenmeyer flask orifice are 40mm and 45mm respectively.
IR. Infrared spectrum (Infrared).
Qnar. The molar flow rate of air passing through the system per hour, in moles per hour (mol/h).
QnCO2.The molar flow rate of CO2 passing through the system per hour, in moles per hour (mol/h).
5 Principles
5.1 General
Under the action of microorganisms in the inoculant, the collagen in the leather or fur sample is degraded to produce CO2.Through elemental analysis and titration the initial carbon content in the sample and the cumulative amount of CO2 produced during the degradation process are measured using the method (or IR method), and the biodegradation rate is calculated.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.
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