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GB/T 41915-2022Nanotechnologies - In vitro MTS assay for measuring the cytotoxic effect of nanoparticles (English PDF)

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Issued by

State Administration for Market Regulation, China National Standardization Administration

Level / Type

National · Recommended

Issue date

October 14, 2022

Implementation date

May 1, 2023

Scope

GB/T 41915-2022 (Nanotechnologies - In vitro MTS assay for measuring the cytotoxic effect of nanoparticles) is available as an English-translated PDF.

GB/T 41915-2022 is the Chinese standard "Nanotechnologies - In vitro MTS assay for measuring the cytotoxic effect of nanoparticles".

Its scope clause reads: This document describes the MTS method for evaluating the effect of nanoobjects and their aggregates and aggregates (NOAA) on cell viability. this Methods include operational requirements and control experiments to determine and analyze the uncertainty of test results.

This document applies to 96-well plates. Its clauses include terms and definitions.

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Document preview — GB/T 41915-2022

National Standard of the People's Republic of China

ICS: 07.120
Classification: C 04

Issued by: State Administration for Market Regulation, China National Standardization Administration

Preface
Introduction
1 Scope
2 Normative references
3 Terms and Definitions
4 Symbols and Abbreviations
5 Material
5.1 Cell Lines
5.2 Detection
5.3 Control
6 Instrument
7 Preparation of Nanoparticle Test Samples
8 Preparation
8.1 Overview
8.2 Medium
8.3 Preparation of Cell Stock
8.4 Validation of cell growth status
8.5 Verifying Reader Consistency
8.6 Preparing the Controlled Experiment
8.6.1 Description of Controlled Experiment
8.6.2 Preparation of CdSO4 stock solution (10mmol/L)
8.6.3 Preparation of Nanoparticle Control Suspension
8.7 Precise dispensing
9 Characterizing the effect of nanoparticles on cell viability
9.1 Overview
9.2 Preparing cell culture plates
9.3 Preparing the Nanoparticle Loading Plate
9.4 Exposure of cells to nanoparticles in cell culture medium
9.5 Treatment of cells with MTS reagent
9.6 Measuring the absorbance of formazan
10 Cell Viability Analysis
11 Interpretation of test results
Appendix A (normative) Alternative cell lines and assays
Appendix B (informative) Case. MTS method based on A549 cell line (EMPA-NIST draft)
Appendix C (informative) Case. MTS method based on Raw264.7 cell line (IANH draft)

1 Scope

This document describes the MTS method for evaluating the effect of nanoobjects and their aggregates and aggregates (NOAA) on cell viability. this

Methods include operational requirements and control experiments to determine and analyze the uncertainty of test results.

This document applies to 96-well plates.

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document.

ISO /T S80004-2 Nanotechnology Terminology Part 2.Nano-objects (Nanotechnologies-Vocabulary-

Part 2.Nano-objects)

3 Terms and Definitions

Terms and definitions defined in ISO /T S80004-2 and the following apply to this document.

3.1 culture vessel

This document uses a 96-well tissue culture grade plate.

Note 1.As long as it meets the requirements of tissue culture grade and is suitable for mammalian cell culture, other well plates (such as 384-well plate, 24-well plate and 6-well plate) can also be used

Used interchangeably.

Note 2.If other sizes of well plates are used in this document, it may

Make necessary adjustments.

[Source. GB/T 16886.5-2017, 3.1]

3.2 dispersion system

A microscopic heterogeneous system in which a discontinuous phase (solid, liquid or gas. discontinuous phase) is dispersed in a continuous phase that differs in composition or state.

Note. If the solid particles are dispersed in a liquid, the dispersion system is called a suspension. If a dispersion system consists of two or more liquid phases, it is called an emulsion liquid. Suspensions consist of solid and liquid phases dispersed in a continuous liquid phase.

3.3 endotoxin

Part of the outer membrane of the extracellular envelope of Gram-negative bacteria.

Note. The main active ingredient is lipopolysaccharide (LPS).

[Source. GB/T 41309-2022, 2.3]

GB/T 36388 | YBB 2027 | YBB 6006 | GB/T 41910 | GB/T 41908 | […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 57 pages — is available in the English PDF.

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