GB/T 41907-2022Assay method of enterokinase activity (English PDF)
肠激酶活性检测方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
October 12, 2022
Implementation date
October 12, 2022
Scope
GB/T 41907-2022 is the English-translated version of 肠激酶活性检测方法.
GB/T 41907-2022 is the Chinese national assay for the activity of enterokinase, the proteolytic enzyme secreted by duodenal cells that cleaves the carboxy-terminal lysine peptide bond preceded by four aspartic acid residues.
Clause 1 says it describes the reagents or materials, the apparatus, the test procedure, the result calculation and the quality control, and clause 3 fixes the unit of measurement: one activity unit (U) is the amount of enzyme required to degrade 95% of 0.5 mg of Thioredoxin-NP-27 to NP-27 within 16 h at 37 °C. Clause 4 calls for analytical grade reagents and has the reagent solutions prepared in large volumes, then subpackaged in small ones and sterilised by autoclaving before use; the preview breaks off inside that clause.
It quotes GB/T 6682 for laboratory water. Issued on 12 October 2022, it has applied since the same date.
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Document preview — GB/T 41907-2022
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- C 27
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword3
- Introduction4
- 1 Scope5
- 2 Normative references5
- 3 Terms and definitions5
- 4 Reagents or materials5
- 5 Apparatus6
- 6 Test procedure6
- 7 Result calculation7
- 8 Quality control7
Foreword
This document is drafted in accordance with GB/T 1.1-2020 "Directives for standardization - Part 1: Rules for the structure and drafting of standardizing documents".
1 Scope
This document describes the reagents or materials, apparatus, test procedure, result calculation, and quality control for the assay method of enterokinase activity.
This document applies to the assay of enterokinase activity.
2 Normative references
The contents of the following documents, through normative references in this text, constitute indispensable provisions of this document. Among them, for dated references, only the edition corresponding to that date applies to this document. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 Enterokinase
A proteolytic enzyme secreted by duodenal cells, which can cleave the lysine carboxy-terminal peptide bond containing 4 aspartic acids (aspartic acid-aspartic acid-aspartic acid-aspartic acid-lysine).
3.2 Activity unit of enterokinase (U)
The amount of enzyme - required to degrade 95% of 0.5 mg of Thioredoxin-NP-27 to NP-27 within 16 h at 37 °C - is 1 activity unit of enterokinase (U).
4 Reagents or materials
Unless otherwise specified, the reagents used in this method shall be of analytical grade.
All reagent solutions should be prepared in large volumes; subpackaged in small volumes and sterilized by autoclaving before use. Reagents that are not suitable for […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
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