GB/T 41212-2021Nanotechnologies -- 5-(and 6)-Chloromethyl-2', 7' Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species(ROS) production in RAW 264.7 macrophage cell line (English PDF)

1 Scope

This document describes how 5-(6)-chloromethyl-2',7'dichlorodihydro-fluorescein diacetate (CM-H2DCF-DA) was detected and evaluated

Methods for the production of reactive oxygen species (ROS) after exposure of RAW264:7 macrophages to nanoobjects, nanoparticles and their aggregates and aggregates:

This document applies to assays using 24-well plates: If other well plates are used, adjust the sample volume and verify the effectiveness of the procedure to

Ensure that test results are credible:

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text: Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document:

ISO 10993-5 Biological evaluation of medical devices - Part 5: In vitro cytotoxicity test (Biological evaluation of medicaldevices-Part 5:Testsforinvitrocytotoxicity)

ISO /T S80004-2 Nanotechnology terminology Part 2: Nano-objects (Nanotechnologies-Vocabulary-Part 2:

Nano-objects)

3 Terms and Definitions

Terms and definitions defined in ISO /T S80004-2, ISO 10993-5 and the following apply to this document:

The following URLs are ISO and IEC responsible for database URLs used in standards:

ISO Online Browsing Platform: http://www:iso:org/obp

IEC online browsing platform: http://www:electropedia:org/

3:1 agglomerate

Stacks, aggregates or mixtures of weakly bound particles whose external surface area is similar to the sum of the surface areas of their individual particles:

Note 1: The forces supporting the aggregates are weak forces, such as van der Waals forces or simple physical entanglement:

Note 2: Aggregates are also referred to as secondary particles, while source particles are referred to as primary particles:

[Source: ISO /T S80004-2:2015, 3:4]

3:2 aggregate aggregate

New particles composed of strongly bound or fused particles may have an external surface area significantly smaller than the sum of their individual particle surface areas:

Note 1: The forces supporting the aggregates are strong forces, such as covalent bonds or originating from sintering or complex physical entanglement:

Note 2: Aggregates are also referred to as secondary particles, while source particles are referred to as primary particles:

[Source: ISO /T S80004-2:2015, 3:5]

3:3 culture vessel culturevessels

Including glass culture dishes, plastic culture flasks or plastic multi-well culture plates and microtiter plates and other vessels suitable for cell culture:

GB/T 36388 | YBB 2027 | YBB 6006 | GB/T 46333 | GB/T 45633 | […]