GB/T 40225-2021Determination of actin antibody—Western blotting method (English PDF)
肌动蛋白抗体的检测 免疫印迹法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
May 21, 2021
Implementation date
December 1, 2021
Scope
GB/T 40225-2021 is the English-translated version of 肌动蛋白抗体的检测 免疫印迹法.
GB/T 40225-2021 is the Chinese national method for detecting actin antibodies by western blotting, the immunoblot technique that separates proteins and then reveals them with a specific antibody.
It applies to the determination of actin antibodies based on this principle. The document defines actin, the globular protein that forms the microfilaments of the cytoskeleton and is often used as an internal reference in cell studies, and the actin antibody raised by immunising animals with it.
It also lists the abbreviations used throughout the method, including APS, BCA, ECL, HRP, NP-40, PBS, PBST, PMSF and PVDF, and refers to GB/T 6682 for the laboratory water.
Laboratories use it to check that an actin antibody binds its target. It was issued on 21 May 2021 and has applied since 1 December 2021.
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Document preview — GB/T 40225-2021
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- A 40
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Abbreviations
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.
1 Scope
This document describes the immunoblot detection method for actin antibodies.
This document is suitable for the determination of actin antibodies based on the principle of western blotting.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 Actin
A type of globular multifunctional protein that forms microfilaments, the main component of the cytoskeleton, is widely expressed in eukaryotic cells.
Note 1. At present, it has been found that there are at least 6 isoforms of actin, which are mainly distributed in the myocardium, skeletal striated muscle and smooth muscle tissues to regulate cell contraction Ability, also expressed in non-muscle cells, regulates cell structure and vitality.
Note 2. The molecular weight of actin is 45ku±3ku (1u~1Da), which is highly conservative and its expression in cells is relatively stable, so it is often used as Correct the internal parameters of the system.
3.2 Actin antibody
Using actin as the antigen, the animal serum obtained after immunization of the animal can bind to actin-specific "antigen-antibody" immune The general term for phytoglobulin.
4 Abbreviations
The following abbreviations apply to this document.
APS. ammonium peroxodisulphate
BCA. bicinchoninic acid
ECL. chemiluminescence reagent (electrogeneratedchemiluminescence)
HRP. horseradish peroxidase (horseradishperoxidase)
NP-40. ethyl phenyl polyethylene glycol (NonidetP40)
PBS. phosphate buffer saline
PBST. Phosphate Tween Buffer (phosphatebuffersalinewithTween)
PMSF. phenylmethylsulfonylfluoride
PVDF. polyvinylidenefluoride
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Normative references
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