GB/T 40192-2021Detection and identification of Colletotrichum Corda using real-time PCR (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document describes the real-time fluorescent PCR quarantine and identification method for the plant pathogenic fungus Colletotrichum spp.

This document is applicable to the detection and screening of Colletotrichum spp. in plants and products that carry Colletotrichum spp.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

SN/T 2589 Test specification for plant pathogenic fungi

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Classification information of Colletotrichum spp

Chinese name. Colletotrichum spp. Scientific name. Colletotrichum Corda.

According to the "Dictionary of the Fungi" (10th edition) (2008) classification system, Colletotrichum spp belongs to the kingdom of Fungi (Fungi). Ascomycota, Sordariomycetes, Glomerelales, Glomerelaceae.

Transmission route. Colletotrichum spp. is mainly spread by seeds or diseased bodies. It can also be spread by wind, rain and insects.

5 Principles of Identification

According to the characteristics of the DNA sequence of Colletotrichum spp., real-time fluorescent PCR method is used to apply the designed primers and probes to Colletotrichum spp. The genus pathogens are tested and identified.

6 Equipment, utensils, reagents and culture medium