GB/T 39730-2020General requirements for cell counting—Flow cytometry (English PDF)

1 Scope

This document describes the terms, definitions, abbreviations, method principles, and reagents or materials, instrument

General requirements for equipment, sample preparation, test procedures, data analysis, method validation and reporting:

This document is applicable to cell counting performed by flow cytometry in research, production and testing:

2 Normative references

There are no normative references in this document:

3 Terms, definitions and abbreviations

3:1 Terms and definitions

The following terms and definitions apply to this document:

3:1:1

Celcounting

Measurement of cell number:

[Source: ISO 20391-1:2018,3:8]

3:1:2

Cell staining

A technique in which specific components of cells are combined with fluorescent dyes or specific antibodies labeled with fluorescent dyes for detection:

Note: The specific components are protein, DNA, RNA, etc:

3:1:3

Certified reference material; CRM Attached with documents issued by an authoritative organization, providing one or more characteristics with uncertainty and traceability obtained using effective procedures

The value of the standard substance:

[Source: JJF1001-2018, 8:15]

3:1:4

Accuracy

Measurementaccuracy

The degree of agreement between the measured value and its true value:

[Source: JJF1001-2018, 5:8, with modification]

3:1:5

Precision

Measurementprecision

Under specified conditions, the degree of agreement between the displayed value or the measured value of the same or similar measured object:

[Source: JJF1001-2018, 5:10, with modification] […]