GB/T 39729-2020General requirements for measurement of cell purity—Flow cytometry (English PDF)

1 Scope

This document describes the terms, definitions, abbreviations, method principles, and reagents or materials, instrument

General requirements for equipment, sample preparation, test procedures, data analysis, method validation and reporting:

This document is applicable to the determination of cell purity by flow cytometry in research, production and testing:

2 Normative references

There are no normative references in this document:

3 Terms, definitions and abbreviations

3:1 Terms and definitions

The following terms and definitions apply to this document:

3:1:1

Target cell celoftestingtarget

Consistent with the detection target, cells with characteristics (such as size, number, and specific markers) that can be detected by flow cytometry:

3:1:2

Cell purity celpurity

The percentage of target cells in the total number of cells in the sample:

3:1:3

Cell staining

A technique in which specific components of cells are combined with fluorescent dyes or specific antibodies labeled with fluorescent dyes for detection:

Note: The specific components are protein, DNA, RNA, etc:

3:2 Abbreviations

The following abbreviations apply to this document:

FSC: Forward Scatter (ForwardScatter)

SSC: Side Scatter (SideScatter)

4 Principle of the method

Using a flow cytometer, the total cells in the sample to be tested are determined by the different optical (scattered light and fluorescence) characteristics of the total cells and the target cells

Distinguish and count the target cells: According to the measured number of total cells and target cells, the purity of the target cells is calculated: