GB/T 38578-2020Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis (English PDF)
水产源致敏性蛋白快速检测 毛细管电泳法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
March 31, 2020
Implementation date
March 31, 2020
Scope
GB/T 38578-2020 is the English-translated version of 水产源致敏性蛋白快速检测 毛细管电泳法.
GB/T 38578-2020 is the Chinese standard "Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis". Its scope clause reads: This standard specifies the method for determination of aquatic allergenic proteins by capillary electrophoresis.
This standard applies to the rapid detection of the content of crustaceous myosin, crustaceous arginine kinase and fish albumin. Its clauses include terms and definitions; principle; reagents or materials.
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Document preview — GB/T 38578-2020
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- A 21
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Principle
- 5 Reagents or materials
1 Scope
This standard specifies the method for determination of aquatic allergenic proteins by capillary electrophoresis.
This standard applies to the rapid detection of the content of crustaceous myosin, crustaceous arginine kinase and fish albumin.
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this article
Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis laboratory specifications and test methods
GB/T 30891 Standard for Sampling of Aquatic Products
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 Allergenic protein
A protein that can cause an allergic reaction in the body.
4 Principle
The allergenic protein in the sample is extracted by protein extract and purified by anion exchange chromatography, measured by capillary electrophoresis, migration time
Qualitative, quantitative by external standard method.
5 Reagents or materials
Unless otherwise specified, only analytical reagents are used.
5.1 Water
Grade 1 water specified in GB/T 6682.
5.2 Methanol
Chromatographically pure.
5.3 Tris-hydroxymethylaminomethane-hydrochloric acid buffer
Trimethylolaminomethane 1.21g, add 900mL of double-distilled water to dissolve, adjust the pH to 7.5 with 6mol/L hydrochloric acid solution, set with distilled water
Make up to 1000mL, filter with 0.45µm filter membrane, ultrasonically shake (power.200W) for 30min, and store at room temperature.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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