GB/T 38421-2019Fur—Detection of animal derived material—Qualitative real-time polymerase chain reaction (PCR) methods (English PDF)
毛皮 源性成分检测 实时荧光定性聚合酶链式反应（PCR）检测方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 31, 2019
Implementation date
July 1, 2020
Scope
GB/T 38421-2019 is the English-translated version of 毛皮 源性成分检测 实时荧光定性聚合酶链式反应（PCR）检测方法.
GB/T 38421-2019 is the Chinese standard "Fur—Detection of animal derived material—Qualitative real-time polymerase chain reaction (PCR) methods". Its scope clause reads: This standard specifies a real-time fluorescent PCR detection method for the qualitative analysis of animal-derived components in natural fur.
This standard applies to the qualitative detection of the origin of eight natural furs (mink, rabbit, mule, raccoon, horse, cow, goat and sheep). Its clauses include terms and definitions, acronyms.
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Document preview — GB/T 38421-2019
National Standard of the People's Republic of China
- ICS
- 59.140.30
- Classification
- Y 46
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and definitions, acronyms
- 3.1 Terms and definitions
- 3.2 Acronyms
1 Scope
This standard specifies a real-time fluorescent PCR detection method for the qualitative analysis of animal-derived components in natural fur.
This standard applies to the qualitative detection of the origin of eight natural furs (mink, rabbit, mule, raccoon, horse, cow, goat and sheep).
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 19495.2 Technical requirements for testing laboratory for genetically modified products
GB/T 21102-2007 Qualitative detection of rabbit-derived components in animal-derived feeds Real-time fluorescent PCR GB/T 25165-2010 Qualitative detection method for bovine, sheep and pig-derived components in gelatin
3 Terms and definitions, acronyms
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1 Real-time polymerase chain reaction
Add a fluorescent group to the polymerase chain reaction system, and use the accumulation of fluorescent signals to monitor the entire PCR process in real time, and
Method of qualitative analysis of unknown template by increasing curve.
Note. Rewrite GB/T 19495.4-2018, definition 3.1.2.
3.1.2 Ct value cyclethreshold
The number of cycles that the fluorescence signal in each reaction tube goes through when it reaches a set threshold.
[GB/T 19495.4-2018, definition 3.1.5]
3.2 Acronyms
The following abbreviations apply to this document.
DNA. deoxyribonucleicacid
DTT. dithiothreitol dNTPs. deoxyribonucleoside triphosphate
FAM. 6-carboxy-fluorescein
Na2EDTA. ethylenediaminetetraaceticaciddisodiumsalt
PCR. polymerase chain reaction
SDS. sodium dodecylsulfate […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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