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GB/T 37870-2019Individual identification by high-throughput sequencing method (English PDF)

个体鉴定的高通量测序方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

August 30, 2019

Implementation date

August 30, 2019

Scope

GB/T 37870-2019 is the English-translated version of 个体鉴定的高通量测序方法.

GB/T 37870-2019 is the Chinese standard "Individual identification by high-throughput sequencing method". Its scope clause reads: This standard specifies the terms and definitions, principles, test conditions, instruments and equipment, reagents, test steps for individual identification of high-throughput sequencing methods.

Analysis of results and results. This standard applies to the human body's blood and blood marks, hair, saliva spots and other samples of individual identification. Its clauses include terms and definitions; abbreviations; principle.

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Document preview — GB/T 37870-2019

National Standard of the People's Republic of China

ICS
07.080
Classification
A 40

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • 1 Scope
  • 2 Normative references
  • 3 Terms and definitions
  • 4 Abbreviations
  • 5 Principle

1 Scope

This standard specifies the terms and definitions, principles, test conditions, instruments and equipment, reagents, test steps for individual identification of high-throughput sequencing methods.

Analysis of results and results.

This standard applies to the human body's blood and blood marks, hair, saliva spots and other samples of individual identification.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.

Pieces. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 29859-2013 Bioinformatics terminology

GB/T 30989-2014 High-throughput gene sequencing technology specification

3 Terms and definitions

The following terms and definitions as defined in GB/T 29859-2013 and GB/T 30989-2014 apply to this document. For convenience

Use, some of the terms and definitions in GB/T 29859-2013 and GB/T 30989-2014 are repeated below.

3.1 Sequencing

The process of determining an amino acid or nucleotide sequence.

[GB/T 29859-2013, definition 2.4.13]

3.2 High-throughput gene sequencing

Different from traditional Sanger (dideoxy) sequencing, it is capable of parallel parallel determination of a large number of nucleic acid molecules.

Often a sequencing reaction can produce sequencing data of no less than 100Mb.

[GB/T 30989-2014, definition 3.19]

4 Abbreviations

The following abbreviations apply to this document.

DNA. Deoxyribonucleic Acid dNTP. deoxy-ribonucleotide triphosphate

PCR. Polymerase Chain Reaction (PolymeraseChainReaction)

SNP. Single Nucleotide Polymorphism (SingleNucleotidePolymorphisms)

STR. Short tandem repeat (ShortTandemRepeat)

5 Principle

After collecting samples, combined with nucleic acid biotechnology and high-throughput sequencing technology to detect specific molecular genetic marker sites (STR locus and […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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