GB/T 36847-2018Detection and identification of Acidovorax cattleyae (English PDF)
兰花褐斑病菌检疫鉴定方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
September 17, 2018
Implementation date
April 1, 2019
Scope
GB/T 36847-2018 is the English-translated version of 兰花褐斑病菌检疫鉴定方法.
GB/T 36847-2018 is the Chinese national method for detecting and identifying Acidovorax cattleyae, the bacterium that causes bacterial brown spot of orchids.
It covers isolation and culture of the pathogen, immunological detection based on the antibody reaction, and molecular detection by PCR and real-time fluorescence PCR on specific DNA sequences, followed by culture traits, biological characteristics and pathogenicity tests.
It applies to quarantine of orchid plants and to field surveys and monitoring of the disease, and refers to SN/T 1157 on quarantine of imported and exported seedlings.
The document gives the basic information on the pathogen, including its synonyms, classification in the Comamonadaceae and its spread over long distances with seedlings and locally through watering, spraying and fertilisation.
It also lists the instruments needed. It was issued on 17 September 2018 and has applied since 1 April 2019.
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Document preview — GB/T 36847-2018
National Standard of the People's Republic of China
- ICS
- 65.020.01
- Classification
- B 16
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Basic information on orchid brown spot disease
- 4 Principle
- 5 Instruments and main reagents
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
1 Scope
This standard specifies the isolation and culture of orchid brown spot pathogen, immunology and molecular biology detection and identification methods.
This standard applies to the quarantine and identification of orchid brown spot disease in orchid plants and the field investigation and monitoring of the disease.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.
Quarantine Regulations for SN/T 1157 Entry and Exit Plant Seedlings
3 Basic information on orchid brown spot disease
Chinese name. Orchid brown spot pathogen, orchid bacterial brown spot disease
Scientific name. Acidovoraxcattleyae (Pavarino1911) Schaadetal.2009
Synonym. Acidovoraxavenaesubsp.cattleyae (Pavarino1911) Wilemsetal.1992 Pseudomonascattleyae (Pavarino1911) Savulescu1947 Bacilluscattleyae (Pavarino) Stapp1928 BacteriumcattleyaePavarino1911 Phytobacteriumcattleyae(Pavarino)Magrou Phytomonascattleyae (Pavarino) Ark
English name. Bacterialbrownspotoforchid
Classification status. Bacteria, Proteobacteria, Betaproteobacteria, Burkholderia Burkholderiales, Comonmonadaceae, Acidovorax.
Transmission route. Long-distance transmission mainly relies on the seedlings, and the short-distance propagation mainly spreads the pathogens by means of foliar watering, spraying or fertilization. Sowing on healthy plants, infecting through leaf wounds and natural orifices, causing secondary infection.
See Appendix A for additional information on orchid brown spot.
4 Principle
Immunological detection of plants or leaves according to the specific reaction between orchid leaf spot and antibody; according to orchid brown spot Specific DNA sequences for molecular biological testing; culture traits, biological characteristics, host range and hazards of orchid leaf spot Isolation, culture and pathogenicity of pathogenic bacteria such as symptoms.
5 Instruments and main reagents
5.1 Instruments and equipment
PCR instrument, real-time fluorescence PCR instrument, microscope, ultra-clean workbench, constant temperature incubator, sterilizer, ice machine, high-speed refrigerated centrifuge, table […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Normative references
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