GB/T 36844-2018Detection and identification of Pseudomonas syringae pv.maculicola (McCulloch) Young et al. (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB 1.1-2009.

1 Scope

This standard specifies the quarantine and identification methods for the bacterial black spot pathogen of Cruciferae.

This standard applies to the quarantine and identification of bacterial black spot pathogens in plant materials such as cruciferous vegetables and their seeds.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 3543.2 Crop seed inspection regulations

GB/T 6682 Analytical laboratory water specifications and test methods

SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products

3 Cruciferous bacterial black spot pathogen information

Chinese name. Cruciferous bacterial black spot disease

Scientific name. Pseudomonassyringaepv.maculicola (McCuloch) Youngetal.

English name. cruciferbacterialblackspot

Taxonomic status. Pseudomonaceae, Pseudomonaceae, Pseudomonas (Pseudomonas), Pseudomonasyringae, a spot-causing species.

See Appendix A for additional information.

4 Principle of the method

Quarantine and identification according to the characteristics of the disease, biological and physiological and biochemical characteristics of the bacteria, molecular biology techniques and pathogenicity.

5 Instruments and appliances

Biological microscope, constant temperature incubator, electronic balance (one thousandth), plant constant temperature light incubator, refrigerator, centrifuge, ultra-clean workbench, PCR amplification instrument, real-time fluorescence quantitative PCR instrument, adjustable pipette, etc.

6 Reagents and media