GB/T 36821-2018Detection and identification of Calonectria ilicicola Boedijn & Reitsma (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard is applicable to the quarantine and identification of peanut black rot fungi of host plants, plant products and soils such as peanuts and soybeans.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.

SN/T 2122-2015 Quarantine sampling method for inbound and outbound plants and plant products

3 Basic information and taxonomic status of peanut black rot

English name of the disease. Black rot of peanut, Red crown rot of soybean

Sexual stage scientific name. Calonectria ilicicola Boedijn

Calonectria ilicicola belongs to Ascomycota, Ascomycetes, Sordariomycetidae, Hypocreales, Nectriacea, Calonectria.

Asexual stage scientific name. Cylindrocladium parasiticum Crous, Wingfield

Cylindrocladium parasiticum belongs to the mitosporic fungi (Mitosporic fungi), Hyphomycetes, Hyphomycetales, Hyphomycetaceae, Cylindrocladium.

Peanut black rot is a typical soil and seed-borne disease with serious damage, rapid spread and difficult to control. The pathogens are widely distributed in the soil. In addition to causing legumes such as peanuts and soybeans, they can also cause leaf spots of plants such as tea trees and oil trees. The pathogen first infects the cotyledons to make them black and rot, which in turn infects the roots and stems of the seedlings. In a humid environment, the disease minister has many molds covering the stem base, stems and leaves lost water and wilted death.

It is often confused with peanut new red-shell rot in the field (Table A.1). Peanut black rot bacteria can grow in the range of 10 °C ~ 30 °C, and the optimum growth temperature is 25 °C ~ 28 °C. See Appendix A for additional information on the distribution of pathogens in the world, host plants, symptoms and comparison with similar diseases.

4 Principle of the method

According to the symptoms, morphological characteristics and PCR-specific amplification of peanut black rot bacteria on the host, the detection and identification were carried out.