GB/T 36004-2018Food contact surfaces cleaning and disinfection efficacy test method—ATP bioluminescence method (English PDF)

3.1 Food contact surfaces

Surfaces such as food packaging materials and containers, and utensils, tableware, equipment, floors, walls in food processing, production and management process that may be in direct contact with food.

3.2 Cleaning

Process of removing the contamination from the item so as to achieve the intended use or further processing requirement.

5.1.5 Precautions

Note the following when using the handheld ATP bioluminescence tester smear method.

a) Do not touch the sampling head and the smearing surface during the smearing process;

b) The operator shall perform a consistent smearing method to obtain repeatable results;

c) Once the sampling is completed, it is recommended to test immediately; once activated, the test shall be carried out within 30 s;

d) The smear sampling rods provided by different manufacturers shall be tested with their own matching instruments. The test results can refer to the standards provided by the instrument supplier. People can be engaged in test after being trained and deemed qualified by the supplier;

e) Different instrument test values cannot be compared together. When using different instruments from different manufacturers, the results may be inconsistent.

5.2 Method 2. benchtop chemiluminescence analyzer test method

5.2.1 Reagents and materials

Unless otherwise stated, only analytically pure reagents and third- or higher-grade water as specified in GB/T 6682 are used in the analysis.

5.2.1.1 ATP test kit. The kit includes cell lysate, assay buffer, fluorescein-luciferase mixture, ATP standard solution.

5.2.1.2 Sterile water. Experimental water after autoclaving. The sterilization condition is. pressure 103 kPa, steam sterilization at 121 °C ~ 126 °C for 20 min. This condition is used for the sterilization operations in this Standard.

5.2.1.3 Autoclaved metal tweezer.

5.2.1.4 Filter paper. CUT the filter paper into 5 cm × 5 cm size and use it after […]

6.1 Instruments

6.1.1 Autoclave.

6.1.2 Constant temperature incubator.

6.1.3 Centrifuge.

6.1.4 Biological safety cabinet.

6.2 Reagents and materials

6.2.1 E. coli strain.

6.2.2 LB liquid medium. Preparation method. ADD 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl into 950 mL of experimental water, SHAKE the container until the solute is dissolved. USE 5 mol/L NaOH to adjust the pH to 7.0, ADD experimental water to 1 L, and CARRY OUT steam sterilization at 103 kPa for 20 min.

6.2.3 Disposable plastic sterile petri dish with a diameter of 9 cm.

6.2.4 Sterilized utility knife.

6.2.5 Sterilized cotton swab.

6.2.6 Sterilized centrifuge tube.

6.3 Artificial containment

6.3.1 Bacterial culture

After activation of the E. coli strain, take 1 mL of E. coli culture medium to inoculate into 100 mL of sterilized LB liquid medium, and culture in a 37 °C incubator at a rate of 220 r/min for 24 h. […]