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GB/T 35910-2018Blocking enzyme-linked immunosorbent assay for detecting the antibody to porcine circovirus type 2 (English PDF)

猪圆环病毒2型阻断ELISA 抗体检测方法

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Issued by

State Administration for Market Regulation, China National Standardization Administration

Level / Type

National · Recommended

Issue date

February 6, 2018

Implementation date

September 1, 2018

Scope

GB/T 35910-2018 is the English-translated version of 猪圆环病毒2型阻断ELISA 抗体检测方法.

GB/T 35910-2018 — This standard specifies the detection of porcine circovirus type 2 antibody blocking ELISA. This standard applies to antibody detection of porcine circovirus disease, vaccine immune antibody monitoring and serum epidemiological survey.

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Document preview — GB/T 35910-2018

National Standard of the People's Republic of China

ICS: 11.220
Classification: B 41

Issued by: State Administration for Market Regulation, China National Standardization Administration

Foreword
1 Scope
2 Normative references
3 Abbreviations
4 instruments, materials and reagents
4.1 Test equipment
4.2 Test Materials

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

This standard proposed by the People's Republic of China Ministry of Agriculture.

This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized.

This standard was drafted. Nanjing Agricultural University.

Drafters of this standard. Jiang Ping, Wang Wei, Bai Juan, Yang Xianglin, Liu Jie.

Porcine circovirus type 2 blocking ELISA

Antibody detection methods

1 Scope

This standard specifies the detection of porcine circovirus type 2 antibody blocking ELISA.

This standard applies to antibody detection of porcine circovirus disease, vaccine immune antibody monitoring and serum epidemiological survey.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB 19489 laboratory biosafety common requirements

NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples

SN/T 2984 inspection and quarantine animal pathogenic microorganisms in biosafety requirements of the experimental activities

3 Abbreviations

The following abbreviations apply to this document.

BSA. Bovine serum albumin

ELISA. Enzyme-linked immunosorbent assay

HRP. horseradishperoxidase

PCV2. Porcine circovirustype 2

TMB. Tetramethylbenzidine

4 instruments, materials and reagents

4.1 Test equipment

4.1.1 enzyme-linked immunosorbent assay.

4.1.2 high-speed desktop refrigerated centrifuge.

4.1.3 micropipette. Specifications 0.5µL ~ 10µL, 20µL ~.200µL, 100µL ~ 1000µL.

4.1.4 12 adjustable micropipette. Size 10µL ~ 100µL.

4.2 Test Materials

4.2.1 96-well enzyme-labeled reaction plate.

4.2.2 Syringe (5 mL).

4.2.3 Microcentrifuge tube (1.5 mL).

4.2.4 Tips (can be matched with pipette).

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.

Referenced standards

Normative references

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