GB/T 35542-2017Taq DNA polymerase (English PDF)
Taq DNA聚合酶
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 29, 2017
Implementation date
July 1, 2018
Scope
GB/T 35542-2017 is the English-translated version of Taq DNA聚合酶.
GB/T 35542-2017 is the Chinese standard "Taq DNA polymerase". Its scope clause reads: This standard specifies the technical requirements TaqDNA polymerase, test methods, packaging, transportation, storage and shelf life.
This standard applies to TaqDNA polymerase extracted from recombinant E. coli. Its clauses include terms and definitions; technical requirements; test methods.
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Document preview — GB/T 35542-2017
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- C 27
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 technical requirements
- 5 test methods
- 6 packaging, transportation and storage
- 7 shelf life
- Appendix A (Normative) TaqDNA polymerase enzyme activity test
- Appendix B (Normative) Exonuclease assay
- Appendix C (Normative) Endonuclease assay
1 Scope
This standard specifies the technical requirements TaqDNA polymerase, test methods, packaging, transportation, storage and shelf life.
This standard applies to TaqDNA polymerase extracted from recombinant E. coli.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 191 Packaging - Pictorial signs.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 Taq DNA polymerase
A thermostable enzyme with a molecular mass of 85 ku derived from recombinant E. coli and containing the full length cloned from Thermus species
TaqDNA polymerase gene. The temperature of 55 °C ~ 110 °C catalytic DNA replication, 74 °C ~ 75 °C for the optimum temperature.
3.2 Taq DNA polymerase activity unit
Using a synthetic hairpin-type oligonucleotide sequence as a template/primer, 1.17 nmol of deoxynucleotides were polymerized into
The amount of enzyme required in double stranded DNA is 1 viable unit (U).
4 technical requirements
4.1 product appearance
Clear transparent liquid, no sediment.
4.2 enzyme activity
>=5000U/mL.
4.3 Impurities
It should not contain exonucleases and endonucleases.
5 test methods
5.1 appearance
Pour the sample directly or into a colorless, transparent test tube and visually observe under natural light conditions.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 17 pages — is available in the English PDF.
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