GB/T 32268-2015Method of performance testing for octadecyl bonded (C18) high performance liquid chromatography (HPLC) column (English PDF)
十八烷基键合相（C18）高效液相色谱柱性能测定方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 10, 2015
Implementation date
July 1, 2016
Scope
GB/T 32268-2015 is the English-translated version of 十八烷基键合相（C18）高效液相色谱柱性能测定方法.
GB/T 32268-2015 sets out how the performance of an octadecyl bonded phase high performance liquid chromatography column is to be measured; the text shortens that object to the C18 HPLC column.
Clause 1 states that the standard specifies the determination of the properties of such columns and that it applies to the determination of C18 HPLC column performance, without naming any exclusion.
Clause 2 cites GB/T 6682, GB/T 9008, GB/T 32267 and JJG 705.
Clause 3 takes over the terms and definitions of GB/T 9008 and GB/T 32267 and then adds its own, for the octadecyl bonded phase (C18) HPLC column, the column internal diameter, the hydrophobic retention factor alphan, which it relates to the number of C18 alkyl chains and to the specific surface area and surface carbon coverage of the filler particles, the hydrophobic selectivity factor alphahp, which it relates to the surface carbon coverage of the filler particles, and the stereoselectivity factor alphass.
The draft shown on the source page carries no contents list and breaks off inside the definition of the stereoselectivity factor, so the clauses holding the measurement procedure itself are not displayed.
The standard was proposed by the Ministry of Science and Technology of the People's Republic of China and is held by the National Instrument Analysis and Testing Standardization Technical Committee (SAC/TC481), with China Association for Instrumental Analysis, Peking University, Beijing Nonferrous Metal Research Institute and Waters Technology (Shanghai) Co., Ltd. among the bodies that took part in the drafting.
It carries ICS 71.040 and Chinese classification N04. It was issued on 10 December 2015 and has applied since 1 July 2016.
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Document preview — GB/T 32268-2015
National Standard of the People's Republic of China
- ICS
- 71.040
- Classification
- N 04
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
1 Scope
This standard specifies the octadecyl bonded phase HPLC column (referred to as the "C18HPLC pillar") determination of properties.
This standard applies to the determination of C18HPLC column performance.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 9008 term liquid chromatography column chromatography and planar chromatography method
GB/T 32267 Determination term performance of the instruments
JJG705 liquid chromatography test procedures
3 Terms and Definitions
GB/T 9008 and GB/T 32267 and defined by the following terms and definitions apply to this document.
3.1 Octadecyl bonded phase (C18) HPLC column
Equipped with octadecyl bonded stationary phase HPLC column.
3.2 Column diameter
Column column pipe inner diameter.
3.3 Hydrophobic retention factor
alphan Characterization C18 stationary phase relative to the number of parameters C18 alkyl chains, and the specific surface area and the surface carbon coverage of filler particles is concerned.
3.4 Hydrophobic selectivity factor
alphahp Characterization of C18 stationary phase was separated parameter substance molecule hydrophobic group selective recognition of, and filler particles surface carbon coverage related.
3.5 Stereoselectivity factor
alphass Parameters characterizing the ability to identify substances to be separated C18 stationary phase molecular shape of the surface of the filler particles with a functional group key degree
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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