GB/T 32112-2015Determination of peroxide in oral care products—High performance liquid chromatography method (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by China National Light Industry Council.

The standard oral care products by the National Technical Committee of Standardization Technical Committee toothpaste (SAC/TC492/SC1) centralized.

1 Scope

This standard specifies the method points Determination of peroxide in oral care products, reagents and reference materials, equipment, Step analysis, the results of the calculation, the recovery rate, standard deviation.

This standard applies to the release of hydrogen peroxide and other peroxide compounds or mixtures (such as carbamide peroxide and zinc peroxide) as raw materials Determination of the peroxide content of oral care products.

This standard peroxide content of hydrogen peroxide meter, the detection limit was 0.3µg/mL, the limit of quantification of 2.0µg/mL. If you take 1.0g Sample, hydrogen peroxide minimum detectable concentration of 30mg/kg, the minimum detectable concentration of 200mg/kg.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 6682 Analysis Laboratory use specifications and test methods

3 Method points

Flooding the sample using extraction reaction releases hydrogen peroxide. A portion of the supernatant was reacted with triphenylphosphine derivatized, derivatized solution was filter through Filter, by liquid chromatography, UV detector, calculated using internal standard method to obtain a sample of hydrogen peroxide content.

4 Reagents and Standards

4.1 Unless otherwise stated in the analysis using only recognized as analytical grade reagents and water GB/T 6682 provided a water.

4.2 acetonitrile. chromatography.

4.3 triphenylphosphine solution. Weigh triphenylphosphine 1.3g, dissolved in acetonitrile, the volume to 25mL, concentration of 0.2mol/L, using now.

4.4 Hydrogen peroxide. 30%, AR.

4.5 3% hydrogen peroxide solution. an amount of 10mL of hydrogen peroxide (30%), water volume to 100mL, should be calibrated before use, calibration party See Appendix B. Method

4.6 Hydrogen peroxide standard stock solution. Weigh a 3% hydrogen peroxide solution (4.5) 3.4g, accurate to 0.0001g, placed in 100mL brown capacity Bottle with water to volume, shake, formulated as a concentration of 1000mg/L of standard stock solution.

4.7 Hydrogen peroxide standard working solution. accurate Pipette stock solution of hydrogen peroxide (4.4), the preparation of water quality concentrations of 5mg/L, 10mg/L, 50mg/L, 100mg/L, 200mg/L, Standard Series 300mg/L working solution.

4.8 diethyl phthalate (DEP). chromatography.

4.9 diethyl phthalate (DEP) internal standard solution. Weigh diethyl phthalate (DEP) (4.8) 0.5g, accurate to 0.0001g, Placed in 500mL flask with acetonitrile to volume, shake. The concentration of 1000mg/L.

4.10 Solvent. preparation of the test solution required acetonitrile and water, acetonitrile first sonication, boiling water was degassed, purged with nitrogen and then processed.