GB/T 28974-2012Detection and identification of Potato virus A-Nanoparticles-enlarged in colloidal gold immunochromatographic assay (English PDF)

1 Scope

This standard specifies the use of colloidal gold nanoparticles sensitizing the immune chromatography detection of potato virus A principle of the experimental procedure and results of judgment

Set and so on.

This standard applies to the organization of potato plants and their products for rapid screening A virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein

Member. For undated references, the latest edition (including any amendments) applies to this document.

GB 19489 General requirements for laboratory biosafety

GB/T 23629-2009 introduction of plant pathogen control technology requirements for safety

GB/T 23632-2009 intercepted entry plant quarantine harmful biological appraisal review procedures

3 Basic information of potato virus A

Chinese name. potato virus A

Name. potatovirusA

Acronym. PVA Taxonomic position. potato virus Y Branch (Potyviridae) genus of potato virus Y (Potyvirus).

For additional information, see Appendix A potato virus A.

4 principle of the method

Based on the principle of colloidal gold immunochromatography double antibody sandwich assay using chloroauric acid and a reducing agent produced gold nanoparticles to conventional

GICA strip processing, improve the detection sensitivity, according to changes in the color of the test strip, the sample is determined whether or not there

Potato A virus.

5 equipment, appliances, materials and reagents