GB/T 28973-2012Detection and identification of Tomato ring spot virus - Nanoparticles-enlarged in colloidal gold immunochromatographic assay (English PDF)
番茄环斑病毒检疫鉴定方法 纳米颗粒增敏胶体金免疫层析法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 31, 2012
Implementation date
June 1, 2013
Scope
GB/T 28973-2012 is the English-translated version of 番茄环斑病毒检疫鉴定方法 纳米颗粒增敏胶体金免疫层析法.
GB/T 28973-2012 is the Chinese standard "Detection and identification of Tomato ring spot virus - Nanoparticles-enlarged in colloidal gold immunochromatographic assay".
Its scope clause reads: This standard specifies the use of nanoparticles sensitizing GICA assay tomato ringspot virus in principle, experimental procedures and results of judgment Set and so on.
This standard applies to plants and their products tissue rapid screening tomato ringspot virus. Its clauses include tomato ringspot virus basic information; principle of the method; equipment, appliances, materials and reagents.
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Document preview — GB/T 28973-2012
National Standard of the People's Republic of China
- ICS
- 65.020.01
- Classification
- B 16
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 tomato ringspot virus basic information
- 4 principle of the method
- 5 equipment, appliances, materials and reagents
1 Scope
This standard specifies the use of nanoparticles sensitizing GICA assay tomato ringspot virus in principle, experimental procedures and results of judgment
Set and so on.
This standard applies to plants and their products tissue rapid screening tomato ringspot virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
GB/T 23629-2009 introduction of plant pathogen control technology requirements for safety
GB/T 23632-2009 intercepted entry plant quarantine harmful biological appraisal review procedures
3 tomato ringspot virus basic information
Chinese name. Tomato ringspot virus
Name. tomatoringspotvirus
Abbreviation. ToRSV
Taxonomic position. cowpea mosaic virus family (Comoviridae) nematodes pass polyhedron virus genus (Nepovirus).
For additional information Tomato ringspot virus in Appendix A.
4 principle of the method
Based on the principle of colloidal gold immunochromatography double antibody sandwich assay using chloroauric acid and a reducing agent produced gold nanoparticles to conventional
GICA strip processing, improve the detection sensitivity, according to changes in the color of the test strip, the sample is determined whether or not there
Tomato ringspot virus.
5 equipment, appliances, materials and reagents
5.1 Equipment
Balance (a sense of volume, 1/10000g), centrifuges, organization stamp mill, low-temperature refrigerator.
5.2 Appliances
Micropipette (2.5µL, 10µL, 20µL, 100µL, 200µL, 1000µL), mortar tubes and the like.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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