GB/T 28068-2011Detection of Xanthomonas citri subsp. Citri using the real-time fluorescent PCR (English PDF)
柑桔溃疡病菌实时荧光PCR检测方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 30, 2011
Implementation date
June 1, 2012
Scope
GB/T 28068-2011 is the English-translated version of 柑桔溃疡病菌实时荧光PCR检测方法.
GB/T 28068-2011 is the Chinese standard "Detection of Xanthomonas citri subsp. Citri using the real-time fluorescent PCR". Its scope clause reads: This standard specifies the citrus canker (Xanthomonascitrisubsp.citri, Xcc) of real-time PCR detection system samples Equipment, detection technology, methods of operation and results criteria.
This standard applies to citrus canker citrus plant material in real-time PCR pathogen detection and disease identification. Its clauses include terms and definitions.
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Document preview — GB/T 28068-2011
National Standard of the People's Republic of China
- ICS
- 65.020.01
- Classification
- B 16
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 3.1 Citrus canker
- 3.2 Citrus canker Xanthomonascitrisubsp.citri
- 3.3 Real-time fluorescence PCR real-timefluorescentPCR
- 3.4 Primer amplification primer
- 3.5 Amplification template templet
- 3.6 Ct values cyclethresholdvalue
1 Scope
This standard specifies the citrus canker (Xanthomonascitrisubsp.citri, Xcc) of real-time PCR detection system samples
Equipment, detection technology, methods of operation and results criteria.
This standard applies to citrus canker citrus plant material in real-time PCR pathogen detection and disease identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 5040 citrus nursery stock quarantine rules
GB/T 19495.2 technical requirements for GMO testing laboratories
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1 Citrus canker
Citrus canker caused by the domestic and foreign citrus quarantine bacterial diseases. Characterized by symptoms of orange leaves, branches and fruit surface
Ulcers appear raised corky lesions, resulting in citrus and deciduous fruit drop, weak grower seriously affected citrus production and fruit quality of appearance.
3.2 Citrus canker Xanthomonascitrisubsp.citri
Refers to cause pathogenic bacteria in Asian citrus canker, Xanthomonas is Xanthomonas Citrus subspecies Combination citrus (Schaad2006;
Young2008).
3.3 Real-time fluorescence PCR real-timefluorescentPCR
Real time polymerase chain reaction method for amplifying a trace amount of a particular DNA fragment in vitro method, since the fluorescence during amplification
Release of substances and can be real-time detection and rapid and sensitive detection of the presence of template DNA.
3.4 Primer amplification primer
Synthetic oligonucleotide sequence, the sequence of the target DNA sequence to be amplified in the same period, for guiding the DNA in vitro
Amplification.
3.5 Amplification template templet
Target sequence to be amplified in vitro DNA amplification used.
3.6 Ct values cyclethresholdvalue
Real time PCR reaction, each reaction tube fluorescent signal reaches a threshold value is set when the number of cycles experienced.
END: Draft Version (GB/T 28068-2011)
GB/T 43165-2023 | GB/T 40135-2021 | GB/T 19495.4-2018 | GB/T 28062-2025 | GB/T 28061-2011 | GB/T 28060-2011 |
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 16 pages — is available in the English PDF.
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