GB/T 27859-2011Chemicals -- Sediment-water chironomid toxicity test -- Spiked sediment method (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

The Standards and Organisation for Economic Co-operation and Development (OECD) Guidelines for the Testing of Chemicals 218 "sediment - water system chironomid toxicity test plus Standard deposit law in the "same (in English) technical content.

This standard configuration and do the following editorial changes.

--- To be consistent with existing national standards, the standard name changed to "chemical sediment - water system chironomid toxicity test plus Standard in sediments Law ";

--- The OECD218 the "introduction" of this standard as "Introduction";

--- The OECD218 in Annex 1 "Terms and definitions" of this standard as Chapter 3, "Terms and definitions." this standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.

This standard was drafted. Jiangsu Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine.

Introduction

The criteria used to assess long-term exposure to chemicals in sediments of freshwater dipteran Chironomus genus (Chironomussp.) Larvae Movies ring. It already Chironomusriparius and toxicity testing Chironomustentans based, above chironomid toxicity test Test method has been in Europe [1-3] and North America [4-8] and established by the comparison test [6, 9]. This standard can also use other existing empirical fully shake Mosquito species, such as Chironomusyoshimatsui [10-11].

Should select the appropriate exposure scenarios depending on the purpose of the test. The standard exposure scenarios are deposits - in the water system of quantitative the test substance added to the sediments, which spiked in the sediment. The exposure scenarios for accumulating sediments simulate the presence of chemicals Level.

Test substance use sediment bioassay generally may be present for a long time in this system. Sediments through biological Multiple routes of exposure of the test substance. The relative importance of each route of exposure, and the exposure time of each toxic effects on the overall contribution, taking Depending on the physical and chemical properties related to chemicals. For substances strongly adsorbed species (such as lgKow > substance 5), or in combination with sediment covalent, let Biological test food intake plus the test substance may be an important route of exposure. In order not to underestimate the toxicity of highly lipophilic substances to test to consider the sediments added to the feed before using the test substance. To all potential exposure pathways into consideration, this standard will focus on Focus on long-term exposure. C.riparius and C.yoshimatsui test duration of 20d ~ 28d, C.tentans of 28d ~ 65d.

If for a particular purpose, short-term data needs, such as toxic effects Unstable chemicals available additional parallel samples were tested, and in the 10d after abandonment.

Final results of the test for the emergence of the total number of adults and emergence time. If you need additional short-term data, it is recommended only appropriate to increase the amount of Parallel test outside after the test was 10d, were measured survival and growth of the larvae.

This standard recommends the use of man made deposits. Compared with natural sediment, formulation has several advantages.

--- Because formulated sediments renewable "standardized matrix" to reduce the experimental uncertainty, and no need to find uncontaminated Dyed clean sediment sources;

--- Test may start at any time, without having to face the seasonal changes in the test sediments, do not pretreated to remove the native fauna. Formulated using deposits also decreased relative to the field to collect a sufficient amount of sediment for routine testing Customs fees;

--- Formulated using sediment toxicity data so that you can compare to each other, thereby performing toxicity classification of substances.

Chemicals sediment - water system chironomid toxicity test Spiked in sediments law

1 Scope

This standard specifies the spiked sediment in sediment assessment method - Test methods for water systems chironomids toxicity.

This standard applies to the assessment of long-term exposure to chemicals in sediments - (. Chironomussp) water freshwater dipteran Chironomus genus Larvae of.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 21809 Chemicals Earthworm Acute Toxicity Test

3 Terms and Definitions

The following terms and definitions apply to this document.

Formulated sediment the mixture was used to simulate the physical composition of natural deposits, also known as reclaimed deposits, artificial or synthetic sediment deposits.

Overlying water the test vessel in the water above the sediment.

Interstitial water interstitialwaterorporewater Water, sediment and soil particles.

Spiked sediment Test test substances have been added by deposits.

Principle 4

The first instar chironomid larvae are exposed to a series of test substances containing different concentrations of sediment - water system tested. First test substance Join sediments until beaker sediment and water aging, the introduction of a chironomid larvae beaker. At the end of testing, measuring Chironomus feather the number and rate of development. If desired, you can also measure the number and quality of the surviving larvae in the 10d after (using the appropriate additional parallel samples). Measured Available experimental data regression analysis to estimate larval survival rates or lead to the emergence or growth rate decreased concentration of x% (eg 15% effective concentration Degree EC15, EC50 EC50, etc.), or statistical hypothesis testing to determine the no-observed-effect concentration (NOEC) or lowest impressive Observed effect concentration (LOEC). The latter need to use statistical tests to the effect with the control values.