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GB/T 27819-2011Chemicals -- Test method of in vitro mammalian cell transformation (English PDF)

化学品体外哺乳动物细胞转化试验方法

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Issued by

Standardization Administration of China

Level / Type

National · Recommended

Issue date

December 30, 2011

Implementation date

August 1, 2012

Scope

GB/T 27819-2011 is the English-translated version of 化学品体外哺乳动物细胞转化试验方法.

GB/T 27819-2011 — This standard specifies the principles of testing chemicals in vitro mammalian cell transformation test, test methods, test data and reports. This standard can be used as an alternative method for detecting the carcinogenic effects of chemicals.

Test Principle 2 Mammalian cell culture test system can be used to detect phenotypic changes caused by chemicals, such a change in their body system malignant Transformation related.

Widely used cell lines include C3H10T1/2,3T3, SHE, Fischer rat cells, the morphological observation of cells, Transformed foci formation or anchorage independence changes in soft agar.

The system is also used for some other students to detect cells after exposure to chemicals Management and morphological changes, but not widely. There is currently no in vitro test for detection of the end proved to have a clear relationship with cancer mechanisms.

Some detection system can be detected cancer-promoting agent.

Test substance for colony formation (colony formation rate) by measuring or cultured cell growth rate of may reflect the influence of the size of its cytotoxicity, it can be calculated by exposure and toxicity associated cytotoxicity evaluation, but because of some trials We need to extend or replace the dish incubation time, therefore, sometimes cytotoxicity data can not be used to calculate the conversion rate. Test Method 3

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Document preview — GB/T 27819-2011

National Standard of the People's Republic of China

ICS: 13.300; 11.100
Classification: A 80

Issued by: Standardization Administration of China

What this standard covers1
Document data2

What this standard covers

This standard specifies the chemical transformation of mammalian cells in vitro test principles, test methods, test data and reports. This standard can be used as the carcinogenic effects of chemicals alternative detection methods.

Document data

International classification (ICS): 13.300; 11.100.

Chinese classification (CCS): A80.

Estimated length of the Chinese original: 6,613 words.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 4 pages — is available in the English PDF.

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